Research On the Induced Resistance of Protein Elicitor PeaT1 with Jinggangmycin in Tobacco Against TMV

doi:10.13560/j.cnki.biotech.bull.1985.2017-0454

Abstract

Abstract: Tobacco virus infects tobacco,tomatoes,peppers,potatoes,and other crops,causing a variety of crop diseases that are difficult to control during production. To explore the optimal mixed proportion of protein elicitor PeaT1 and Jinggangmycin to defense tobacco virus disease,this study chosen Nicotiana benthamiana cv. Huangmiaoyu having 3 to 7 leaves,respectively by spraying water,Jinggangmycin,and combination of PeaT1 and PeaT1-Jinggangmycin. Virus TMV-GFP was inoculated by the way of friction,and viral infection situation was observed under UV light. The results showed that the combination of Jinggangmycin mixed with PeaT1 had better synergism than using single one;PeaT1：Jinggangmycin at 3：4 mixed ratio had the most cost-efficient,and cotoxicity coefficient reached 157.64,the relative control effect reached 73.39%. Therefore,we thought that Jinggangmycin and PeaT1 both generated tobacco resistance against TMV,and their combination had synergetic effect.

Key words: protein elicitor, Jinggangmycin, induced resistance, TMV-GFP

PENG Zhi-chao, TONG Zan-hua, QIU De-wen. Research On the Induced Resistance of Protein Elicitor PeaT1 with Jinggangmycin in Tobacco Against TMV[J]. Biotechnology Bulletin, 2017, 33(11): 92-95.

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