Screening and Study of a New Promoter with Effective Expression of Alkaline Protease

doi:10.13560/j.cnki.biotech.bull.1985.2017-0748

Abstract

Abstract: The alkaline protease has extensive applications but in short supply,for which the efficient expression system of alkaline protease is constructed. A new type of promoter pChi was screened from the Bacillus licheniformis by SDS-PAGE and mass spectrometric detection. With the other two strong constitutive promoters pShuttle-09 and pyxiE as the control,pChi’s expression activities in the alkaline proteases from B. clausii and B. licheniformis was investigated. Further,six types of recombinant expression vectors and bacteria were constructed. The results showed that the functional expression of pChi was 1.25 times of pShuttle-09 and 2 times of pyxiE,which offers the foundation for the gene heterologous expression in mediated B. subtilis expression system.

Key words: alkaline protease, efficient expression system, promoter, heterologous expression

CHEN Kun, YUAN Fei-yan, CHAI Hao-nan, LIU Huan, WANG Xing-ji, ZHANG Hui-tu, LU Fu-ping. Screening and Study of a New Promoter with Effective Expression of Alkaline Protease[J]. Biotechnology Bulletin, 2018, 34(1): 208-214.

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