Preparation and Identification of Polyclonal Antibody Against Calponin from Helicoverpa armigera

doi:10.13560/j.cnki.biotech.bull.1985.2017-1115

Abstract

Abstract: Polyclonal antibodies against calponin from Helicoverpa armigera（HaCal）were prepared by protein immunization and used for preliminary application. The HaCal was expressed by the prokaryotic expression system,purified by Ni-column,and then immunized the mice by footpad and subcutaneous injection. The titer of polyclonal antibody against HaCal protein was determined by ELISA after four immunizations. The immunological specificity of the polyclonal antibody was detected by Western blot and immunohistochemical staining. The titer of mouse anti-HaCal polyclonal antibody prepared by protein immunization was higher than 1：819 200. Western blot and immunohistochemical staining showed that the antibody specifically recognized the native HaCal protein in the H. armigera. In summary,the polyclonal antibody against HaCal with high titer and specificity was prepared by protein immunization,which provided an important experimental tool for further studying the expression characteristics of HaCal protein and clarifying how to invovle in drug resistance of H. armigera.

Key words: Helicoverpa armigera, calponin, polyclonal antibody

Nijiati·DILIMULATI, ZHAO Jie, AI Xin-yu, LIU Xiao-ning. Preparation and Identification of Polyclonal Antibody Against Calponin from Helicoverpa armigera[J]. Biotechnology Bulletin, 2018, 34(7): 154-159.

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