Antibodies Preparation and the Establishment of Chemiluminescence Immunoassay Detection Method for Cystain C Polypeptide

doi:10.13560/j.cnki.biotech.bull.1985.1017-1084

Biotechnology Bulletin ›› 2018, Vol. 34 ›› Issue (8): 75-79.doi: 10.13560/j.cnki.biotech.bull.1985.1017-1084

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Antibodies Preparation and the Establishment of Chemiluminescence Immunoassay Detection Method for Cystain C Polypeptide

WANG Yun-long^1,2,3, XU Rui-fang¹, LI Yu-lin^2,3, WANG Ji-chuang^2,3, CHENG Chun-jie³, GAO Yu-hong³

1. School of Life Sciences,Zhengzhou University,Zhengzhou 450001;
2. Henan Bioengineering Technology Research Center,Zhengzhou 450001;
3. Henan Engineering Laboratory for Biomarker Quantitative Detection,Zhengzhou 450001

Received:2017-12-18 Online:2018-08-26 Published:2018-09-04

Abstract

Abstract: The objective of this work is to prepare cystain C antibody and establish the chemiluminescence detection method for the early diagnosis of renal damnification. Cystatin C monoclonal antibody was prepared through chemical synthesis of cystain C polypeptide,then competitive inhibition assay was used for preliminary screening of paired antibody,and chemiluminescence immunoassay detection method of cystain C was established. Totally,39 strains of cystatin C monoclonal antibody cells were obtained,and paired antibody was screened. By analyzing the linear range,minimum detection limit,precision,accuracy and other performance evaluation analysis,chemical luminescence detection method for detecting cystatin C was established,with linear range of 50--20 000 ng/mL and the minimum detection limit of 3 ng/mL. Conclusively,cystatin C paired antibody was prepared and chemiluminescence detection method was established successfully,which provides a new detection technique and method for early renal damnification.

Key words: cystatin C polypeptide, antibody paired, chemiluminescence

WANG Yun-long, XU Rui-fang, LI Yu-lin, WANG Ji-chuang, CHENG Chun-jie, GAO Yu-hong. Antibodies Preparation and the Establishment of Chemiluminescence Immunoassay Detection Method for Cystain C Polypeptide[J]. Biotechnology Bulletin, 2018, 34(8): 75-79.

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Antibodies Preparation and the Establishment of Chemiluminescence Immunoassay Detection Method for Cystain C Polypeptide

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