Increase of Flavonoids Content in Arabidopsis Heterologous Expressing CiCHI Gene

doi:10.13560/j.cnki.biotech.bull.1985.2019-0669

Abstract

Abstract: Chalcone isomerase（CHI）is the second key enzyme in flavonoids synthesis pathway,and plays an important role in regulating the whole metabolic pathway. Clarifying the function of CiCHI would provide the theoretical evidences for further revealing the anti-stress mechanism of Caragana intermedia. Degenerate primers were designed according to the conserved domain of known CHI genes,and the full length of CiCHI gene sequence was cloned using C. intermedia cDNA as template,then was confirmed by sequence and phylogenetic analysis. The transcript level of CiCHI was induced by ultraviolet（UV）,NaCl and abscisic acid（ABA）treatments as analyzed by quantitative real-time PCR（qRT-PCR）. The expression of CiCHI was the highest in leaves,relatively high in stems,and the lowest in roots. Heterologous expression of CiCHI significantly increased the content of total flavonoids in transgenic Arabidopsis. All these results confirmed that CiCHI was functional in transgenic Arabidopsis.

Key words: chalcone isomerase（CHI）, Caragana intermedia, Flavonoids content, transgenic Arabidopsis

YANG Fei-yun, BAI Jie, LIU Kun, WANG Rui-gang. Increase of Flavonoids Content in Arabidopsis Heterologous Expressing CiCHI Gene[J]. Biotechnology Bulletin, 2019, 35(11): 39-45.

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