Synergistic Effect of Musca domestica Cecropin and Gentamicin Against Bacillus proteus vulgaris

doi:10.13560/j.cnki.biotech.bull.1985.2020-0245

Abstract

Abstract:

The combined antibacterial action and the synergistic antibacterial mechanism of Musca domestica cecropin（MDC）and gentamicin on Bacillus proteus vulgaris were investigated. Micro broth dilution,chessboard,and crystal violet staining were used to study the synergistic antibacterial and antibiofilm effects of the two drugs. Fluorescence staining,flow cytometry and transmission electron microscopy（TEM）were employed to observe the antibacterial mechanism of the combination of the two drugs. The fractional inhibitory concentration of MDC and gentamicin was 0.313,indicating that the 2 drugs presented promising synergistic antibacterial effect. According to the results of crystal violet staining,the two drugs demonstrated a synergistic effect on the initial biofilm,mature biofilm and extracellular protein of the Bacillus proteus vulgaris. The results of fluorescence and flow cytometry showed that the two drugs efficiently killed bacteria and increased the number of bacterial deaths. TEM observation revealed that most bacterial cell walls were damaged,their cell membranes were incomplete,and intracellular necrosis caused cell swelling and rupture. The MDC and gentamicin act synergistically against Bacillus proteus vulgari,and the mechanism of the two drugs is complementary.

Key words: Bacillus proteus vulgaris, Musca domestica cecropin, gentamicin, synergistic antibacterial action

CHEN Qing-ru, GUI Shui-qing, LU Xue-mei. Synergistic Effect of Musca domestica Cecropin and Gentamicin Against Bacillus proteus vulgaris[J]. Biotechnology Bulletin, 2020, 36(11): 85-93.

Figures/Tables 11

References 21

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