Biotechnology Bulletin ›› 2020, Vol. 36 ›› Issue (12): 98-103.doi: 10.13560/j.cnki.biotech.bull.1985.2020-0497
Previous Articles Next Articles
Received:
2020-04-28
Online:
2020-12-26
Published:
2020-12-22
Contact:
DU Cai-ping
E-mail:mengli@xzhmu.edu.cn;caipingdu@xzhmu.edu.cn
MENG Li, DU Cai-ping. Eukaryotic Expression,Purification and Activity Identification of Rat His-Akt1 Recombinant Protein[J]. Biotechnology Bulletin, 2020, 36(12): 98-103.
引物名称 | 引物序列(5'-3') | 酶切位点 |
---|---|---|
His-Akt1上游 | CCCAAGCTTGGGATGCATCATCATCAT- CATCACATGAACGACGTAGCCATTGT | Hind III |
His-Akt1下游 | GAATTCTCAGGCTGTGCCACTGGCT- GAGTC | EcoR I |
引物名称 | 引物序列(5'-3') | 酶切位点 |
---|---|---|
His-Akt1上游 | CCCAAGCTTGGGATGCATCATCATCAT- CATCACATGAACGACGTAGCCATTGT | Hind III |
His-Akt1下游 | GAATTCTCAGGCTGTGCCACTGGCT- GAGTC | EcoR I |
[1] | Santi SA, Lee H. The Akt isoforms are present at distinct subcellular locatios[J]. Am J Physiol Cell Physiol, 2010,298(3):580-591. |
[2] | Balasuriya N, Mckenna M, Liu X, et al. Phosphorylation-dependent inhibition of Akt1[J]. Genes(Basel), 2018,9(9):450. |
[3] |
Lee MY, Luciano AK, Ackah E, et al. Endothelial Akt1 mediates angiogenesis by phosphorylating multiple angiogenic substrates[J]. Proc Natl Acad Sci USA, 2014,111(35):12865-12870.
URL pmid: 25136137 |
[4] | Kim MY, Park JY, Park HS. Akt1-mediated phosphorylation of RBP-Jk controls notch1 signaling[J]. Biochemistry(Mosc), 2019,84(12):1537-1546. |
[5] |
Marte BM, Downward J. PKB/Akt:connecting phosphoinositide 3-kinase to cell survival and beyond[J]. Trends Biochem Sci, 1997,22(9):355-358.
URL pmid: 9301337 |
[6] |
Hutchinson JN, Jin J, Cardiff RD, et al. Activation of Akt1(PKB-alpha)canaccelerate ErbB-2 mediated mammary tumorigenesis but suppresses tumor invasion[J]. Cancer Res, 2004,64(9):3171-3178.
URL pmid: 15126356 |
[7] |
Nileeka B, Maya TK, Liu X, et al. Genetic code expansion and live cell imaging reveal that Thr-308 phosphorylation is irreplaceable and sufficient for Akt1 activity[J]. J Biol Chem, 2018,293(27):10744-10756.
URL pmid: 29773654 |
[8] |
Manning BD, Toker A. AKT/PKB signaling:Navigating the network[J]. Cell, 2017,169(3):381-405.
URL pmid: 28431241 |
[9] |
Risso G, Blaustein M, Pozzi B, et al. Akt/PKB:one kinase, many modifications[J]. Biochem J, 2015,468(2):203-214.
URL pmid: 25997832 |
[10] | Wang S, Huang X, Sun D, et al. Extensive crosstalk between O-GlcNAcylation and phosphorylation regulates Akt signaling[J]. PLoS One, 2012,7(5):e37427. |
[11] |
Guo J, Chakraborty AA, Liu P, et al. pVHL suppresses kinase activity of Akt in a proline-hydroxylation-dependent manner[J]. Science, 2016,353(6302):929-932.
doi: 10.1126/science.aad5755 URL pmid: 27563096 |
[12] |
Yuichiro Y, Takehiro S, Yo M. SMYD3-mediated lysine methylation in the PH domain is critical for activation of AKT1[J]. Oncotarget, 2016,7(46):75023-75037.
doi: 10.18632/oncotarget.v7i46 URL |
[13] |
Yang WL, Wu CY, Wu J, et al. Regulation of Akt signaling activation by ubiquitination[J]. Cell Cycle, 2010,9(3):487-497.
URL pmid: 20081374 |
[14] |
Lin CH, Liu SY, Lee EH. SUMO modification of Akt regulates global SUMOylation and substrate SUMOylation specificity through Akt phosphorylation of Ubc9 and SUMO1[J]. Oncogene, 2016,35(5):595-607.
URL pmid: 25867063 |
[15] |
de la Cruz-Herrera CF, Campagna M, Lang V, et al. SUMOylation regulates AKT1 activity[J]. Oncogene, 2015,34(11):1442-1450.
URL pmid: 24704831 |
[16] |
Li R, Wei J, Jiang C, et al. Akt SUMOylation regulates cell proliferation and tumorigenesis[J]. Cancer Res, 2013,73(18):5742-5753.
URL pmid: 23884910 |
[17] |
Sarbassov DD, Guertin DA, Ali SM, et al. Phosphorylation and regulation of Akt/PKB by the rictor-mTOR complex[J]. Science. 2005,307(5712):1098-1101.
URL pmid: 15718470 |
[18] |
Landgraf KE, Pilling C, Falke JJ. Molecular mechanism of an oncogenic mutation that alters membrane targeting:Glu17 Lys modifies the PIP lipid specificity of the AKT1 PH domain[J]. Biochemistry, 2008,47:12260-12269.
URL pmid: 18954143 |
[19] |
Yang G, Murashige DS, Humphrey SJ, et al. A positive feedback loop between Akt and mTORC2 via SIN1 phosphorylation[J]. Cell Rep, 2015,12(6):937-943.
URL pmid: 26235620 |
[20] |
Balu DT, Carlson GC, Talbot KK, et al. Akt1 deficiency in schizophrenia and impairment of hippocampal plasticity and function[J]. Hippocampus, 2012,22(2):230-240.
doi: 10.1002/hipo.20887 URL pmid: 21049487 |
[21] |
Zheng W, Wang H, Zeng Z, et al. The possible role of the Akt signaling pathway in schizophrenia[J]. Brain Res, 2012,1470:145-158.
URL pmid: 22771711 |
[22] | Wu W, Chen Y, Huang L, et al. Effects of AKT1 E17K mutation hotspots on the biological behavior of breast cancer cells[J]. Int J Clin Exp Pathol, 2020,13(3):332-346. |
[23] |
Nitulescu GM, Margina D, Juzenas P, et al. Akt inhibitors in cancer treatment:The long journey from drug discovery to clinical use(Review)[J]. Int J Oncol, 2016,48(3):869-885.
URL pmid: 26698230 |
[24] | Oeck S, Al-Refae K, Riffkin H, et al. Activating Akt1 mutations alter DNA double strand break repair and radiosensitivity[J]. Scientific Report, 2017,7:42700. |
[1] | QIN Xue-jing, WANG Yu-han, CAO Yi-bo, ZHANG Ling-yun. Prokaryotic Expression and Preparation of Polyclonal Antibody of PwHAP5 Gene in Picea wilsonii [J]. Biotechnology Bulletin, 2022, 38(8): 142-149. |
[2] | YI Fang, LAI Peng-cheng, ZHENG Xi-ao, HU Shuai, GAO Yan-li. Research on the Preparation and Purification of Kod DNA Polymerase [J]. Biotechnology Bulletin, 2022, 38(5): 183-190. |
[3] | WANG Qiao-ju, HU Yu-meng, WEN Ya-ya, SONG Li, MENG Chuang, PAN Zhi-ming, JIAO Xin-an. Expression and Activity Identification of SARS-CoV-2 S1 Protein [J]. Biotechnology Bulletin, 2022, 38(3): 157-163. |
[4] | WU Kun-kun, XU Xing, JI Ce, REN Jian-feng, LI Wei-ming, ZHANG Qing-hua. Eukaryotic Expression Vector Construction of Danio rerio notch3 Gene and Its Expression Analysis [J]. Biotechnology Bulletin, 2022, 38(1): 179-186. |
[5] | HU Xiao, WANG Bao-bao, DOU Shao-hua, JIANG Nan, FU Chang-zhen, JIN Hang, GAO Feng-shan. Construction of a Eukaryotic Expression Vector of SLA-2 Gene from Yantai Black Pigs and Its Expression [J]. Biotechnology Bulletin, 2021, 37(10): 143-151. |
[6] | TANG Lu, DONG Li-ping, YIN Mo-li, LIU Lei, DONG Yuan, WANG Hui-yan. Preparation and Identification of a Novel FGF20 Monoclonal Antibody [J]. Biotechnology Bulletin, 2021, 37(10): 179-185. |
[7] | ZHOU Yang, WANG Tao-tao, YAN Dan-dan, WANG Ying-ying, SHI Qin-xuan, SUN Li-hui, LIN Feng. Advances in Biotechnological Application of Elastin-like Polypeptides as Functional Nanomaterials [J]. Biotechnology Bulletin, 2020, 36(11): 198-208. |
[8] | YANG Ling-qin, LIU Jing, LI Wei, DAI Liang-ying. Research Advances in Potassium Ion Channel AKT1 in Plant [J]. Biotechnology Bulletin, 2019, 35(4): 94-100. |
[9] | LI Ling, BAI Juan-juan, GUO Mei, WANG Si-yu, WANG Xiao-yue. Prokaryotic Expression,Purification and Antibody Preparation of Transcription Factor CNR in Tomato Fruit [J]. Biotechnology Bulletin, 2019, 35(2): 1-8. |
[10] | ZHANG Peng-fei, BIAN Shuai, ZHAO Yue, ZHAO Yu, WANG Jia-wen. Cloning,Prokaryotic Expression and Activity Research of Panax ginseng Thionins [J]. Biotechnology Bulletin, 2019, 35(2): 53-57. |
[11] | CHEN Jian-jun, LIU Liang-tao, CAO Xiang-lin. Cloning,Expression and Enzyme Production of Laccase Gene lac1680 in Phanerochaete chrysosporium [J]. Biotechnology Bulletin, 2018, 34(4): 214-220. |
[12] | ZHAI Xiao-xin, GAO Hua, JIANG Ping, XU Chong-bo, ZHANG Zong-hui, LI Wen-zhe, GAO Feng-shan. Construction of Eukaryotic Expression Vector of SLA-2-HB01 from Hebao Pigs and Its Expression in PK15 Cells [J]. Biotechnology Bulletin, 2018, 34(12): 132-139. |
[13] | WANG Peng-ju,TAN Huan-bo,SU Wen-cheng,ZHANG Wen-yu,ZOU Pei-jian. Study on Refolding and Purification of Plectasin MP1106 [J]. Biotechnology Bulletin, 2017, 33(9): 94-100. |
[14] | YUAN Min, QI Yu-rong ,WANG Rui-ju. Purification and Activity Analysis of Tag-free Protein Kinase BIN2 [J]. Biotechnology Bulletin, 2017, 33(7): 145-149. |
[15] | WANG Ya-mei WEI Yuan-jie AI Xin-yu LIU Xiao-ning. Isolation and Purification of His-CYP6J1 Fusion Protein from Aphis gossypii and Preparation of Its Polyclonal Antibody [J]. Biotechnology Bulletin, 2017, 33(5): 164-169. |
Viewed | ||||||
Full text |
|
|||||
Abstract |
|
|||||