Biotechnology Bulletin ›› 2022, Vol. 38 ›› Issue (9): 264-270.doi: 10.13560/j.cnki.biotech.bull.1985.2021-1529
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HU Hai-yang1(), YING Wan-qin1, HE Jun2, LV Zhi-xian1, XIE Xiao-ping3(), DENG Zhong-liang1()
Received:
2021-12-09
Online:
2022-09-26
Published:
2022-10-11
Contact:
XIE Xiao-ping,DENG Zhong-liang
E-mail:574087481@qq.com;443661273@qq.com;dzl021015@163.com
HU Hai-yang, YING Wan-qin, HE Jun, LV Zhi-xian, XIE Xiao-ping, DENG Zhong-liang. Establishment and Application of ERA Real-time Fluorescence Method for Rapid Detection of Mycoplasma pneumoniae[J]. Biotechnology Bulletin, 2022, 38(9): 264-270.
名称 Name | 序列 Sequence(5'-3') | 长度 Length/bp |
---|---|---|
Forward Primer 1(F1) | TTGAAGGTGAAGAACGCCG- AGGCGGACAC | 29 |
Forward Primer 2(F2) | CGAAGAGCAATGAAAAACTC- CAGGGCGCTGA | 31 |
Reverse Primer 1(R1) | GGACTGACCCGACTCCTCGCT- CCGCTTAATG | 31 |
Reverse Primer 2(R2) | ATTGGAGTTGCCGCCTGATCC- CGAACTGGAA | 31 |
Probe | TGAAAAAGAAATCGGACTCG- GAGGACAA[FAM-dT]G [THF][BHQ1-dT]CAGCTG- CAGTTAGAA3'Block | 46 |
Table 1 Primer and probe sequences of ERA real-time fluorescence method
名称 Name | 序列 Sequence(5'-3') | 长度 Length/bp |
---|---|---|
Forward Primer 1(F1) | TTGAAGGTGAAGAACGCCG- AGGCGGACAC | 29 |
Forward Primer 2(F2) | CGAAGAGCAATGAAAAACTC- CAGGGCGCTGA | 31 |
Reverse Primer 1(R1) | GGACTGACCCGACTCCTCGCT- CCGCTTAATG | 31 |
Reverse Primer 2(R2) | ATTGGAGTTGCCGCCTGATCC- CGAACTGGAA | 31 |
Probe | TGAAAAAGAAATCGGACTCG- GAGGACAA[FAM-dT]G [THF][BHQ1-dT]CAGCTG- CAGTTAGAA3'Block | 46 |
阳性 Positive | 阴性 Negative | 合计 Total | |
---|---|---|---|
荧光定量PCR法 Fluorescence qPCR method | 26 | 8 | 34 |
ERA实时荧光法 ERA real-time fluorescence method | 25 | 9 | 34 |
Table 2 Comparison between ERA real-time fluorescence method and fluorescence qPCR method
阳性 Positive | 阴性 Negative | 合计 Total | |
---|---|---|---|
荧光定量PCR法 Fluorescence qPCR method | 26 | 8 | 34 |
ERA实时荧光法 ERA real-time fluorescence method | 25 | 9 | 34 |
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