Biotechnology Bulletin ›› 2023, Vol. 39 ›› Issue (1): 305-314.doi: 10.13560/j.cnki.biotech.bull.1985.2022-0408

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Novel Coronavirus Subunit Vaccine and Screening of Its Efficient Immune Enhancer

WANG Xiang-kun1(), SONG Xue-hong2, LIU Jin-long3, GUO Pei-hong4, ZHUANG Xiao-feng3, WEI Liang-meng1, ZHOU Fan1, ZHANG Shu-yu1, GAO Pan-pan1, WEI Kai1()   

  1. 1. College of Animal Science and Technology, Shandong Agricultural University, Tai'an 271018
    2. College of Basic Medicine and Bioscience, Suzhou University, Suzhou 215123
    3. Shandong Beiruikang Biotechnology Co., Ltd., Weifang 261061
    4. Kunshan Beruikang Biotechnology Co., Ltd., Kunshan 215345
  • Received:2022-04-03 Online:2023-01-26 Published:2023-02-02
  • Contact: WEI Kai E-mail:1765141493@qq.com;weikaisdau@163.com

Abstract:

This work aims to promote the development of novel coronavirus(2019-nCoV)subunit vaccine and explore the screening of high-efficiency immune enhancers suitable for the vaccine. We induced the expressions of 2019-nCoV-S and 2019-nCoV-N proteins respectively, determined the contents of the target protein after purification, different concentrations of pine pollen polysaccharide(PPPS)(200, 400, 800 mg/mL)were added as adjuvants to prepare genetic engineering subunit vaccine, and astragalus polysaccharide adjuvant was used as control. One hundred SPF BALB/c mice were randomly divided into ten groups and each subunit vaccine was used in five groups. After immunization, the immune indexes of mice in each group were detected to explore the immune enhancement effect of PPPS on 2019-nCoV-S and 2019-nCoV-N subunit vaccines. The results showed that the recombinant expression and purification of the target protein showed a single band at 55.68 and 45.64 kD, respectively, and the expression was correct. The protein concentration was 1.12 and 0.66 mg/mL, respectively. After immunizing mice with the adjuvant subunit vaccine, it was found that 400 mg/mL PPPS significantly improve the immune effect of the two vaccines, and the effect was better than that of astragalus polysaccharide adjuvant. In summary, the two recombinant proteins can induce higher antibody levels, and PPPS can be used as a candidate adjuvant for 2019-nCoV subunit vaccine.

Key words: novel coronavirus, genetically engineered subunit vaccine, immune adjuvant, pine pollen polysaccharide