Biotechnology Bulletin ›› 2023, Vol. 39 ›› Issue (1): 305-314.doi: 10.13560/j.cnki.biotech.bull.1985.2022-0408
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WANG Xiang-kun1(), SONG Xue-hong2, LIU Jin-long3, GUO Pei-hong4, ZHUANG Xiao-feng3, WEI Liang-meng1, ZHOU Fan1, ZHANG Shu-yu1, GAO Pan-pan1, WEI Kai1()
Received:
2022-04-03
Online:
2023-01-26
Published:
2023-02-02
Contact:
WEI Kai
E-mail:1765141493@qq.com;weikaisdau@163.com
WANG Xiang-kun, SONG Xue-hong, LIU Jin-long, GUO Pei-hong, ZHUANG Xiao-feng, WEI Liang-meng, ZHOU Fan, ZHANG Shu-yu, GAO Pan-pan, WEI Kai. Novel Coronavirus Subunit Vaccine and Screening of Its Efficient Immune Enhancer[J]. Biotechnology Bulletin, 2023, 39(1): 305-314.
Fig. 1 Induced expressions and purifications of recombinant protein 2019-nCoV-S and 2019-nCoV-N M, Marker. A: 1, Total cell protein of pET28a(+)-2019-nCoV-S/BL21 strain 1 before induction. 2, Total cell protein pET28a(+)-2019-nCoV-S/BL21 strain 1 after induction. 3, Total cell protein of pET28a(+)-2019-nCoV-S / BL21 strain 2 before induction. 4, Total cell protein of pET28a(+)-2019-nCoV-S/BL21 strain after induction. 2. 5, Total cell protein of pET28a(+)-2019-nCoV-N/BL21 strain 1 before induction; 6, Total cell protein of pET28a(+)-2019-nCoV-N/ L21 strain 1 after induction. 7, Total cell protein of pET28a(+)-2019-nCoV-N / BL21 strain 2 before induction. 8,Total cell protein of pET28a(+)-2019-nCoV-N /BL21 strain 2 after induction. B: 1, The purified recombinant protein 2019-nCoV-S was sampled at 20 μL. 2, The purified recombinant protein 2019-nCoV-S was sampled at 10 μL. C: 1, The purified recombinant protein 2019-nCoV-N was sampled at 20 μL. 2, The purified recombinant protein 2019-nCoV-N was sampled at 10 μL
Fig. 3 Determination of specific IgG antibody after immu-nization with vaccine A: Changes of 2019-nCoV-S subunit vaccine on the content of IgG in serum. B: Changes of 2019-nCoV-N subunit vaccine on the content of IgG in serum. Shoulder injection of the same lowercase letters indicates no significant difference(P > 0.05), different lowercase letters indicate significant differences(P < 0.05), and different uppercase letters indicate extremely significant differences(P < 0.01), the same below
Fig. 4 Changes of IL-2 content in serum after immuniza-tion with vaccine A: Changes of IL-2 content in serum after immunization with 2019-nCoV-S subunit vaccine. B: Changes of IL-2 content in serum after immunization with 2019-nCoV-N subunit vaccine.
Fig. 5 Changes of peripheral blood lymphocyte conversion rate after immunization with vaccine A: Changes of peripheral blood lymphocyte conversion rate after immunization with 2019-nCoV-S subunit vaccine. B: Changes of peripheral blood lymphocyte conversion rate after immunization with 2019-nCoV-N subunit vaccine.
Fig. 6 Determination of CD4+T and CD8+T cell content in peripheral blood after immunization with vaccine A: Changes of CD4+T cell content in blood after immunization with 2019 n-CoV-S subunit vaccine. B: Changes of CD8+T cell content in blood after immunization with 2019 n-CoV-S subunit vaccine. C: Changes of CD4+T cell content in blood after immunization with 2019 n-CoV-N subunit vaccine. D: Changes of CD8+T cell content in blood after immunization with 2019 n-CoV-N subunit vaccine.
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