Biotechnology Bulletin ›› 2023, Vol. 39 ›› Issue (9): 117-125.doi: 10.13560/j.cnki.biotech.bull.1985.2023-0090
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WANG Zi-ying(), LONG Chen-jie, FAN Zhao-yu, ZHANG Lei()
Received:
2023-02-07
Online:
2023-09-26
Published:
2023-10-24
Contact:
ZHANG Lei
E-mail:747262414@qq.com;arabilab@whu.edu.cn
WANG Zi-ying, LONG Chen-jie, FAN Zhao-yu, ZHANG Lei. Screening of OsCRK5-interacted Proteins in Rice Using Yeast Two-hybrid System[J]. Biotechnology Bulletin, 2023, 39(9): 117-125.
Fig. 1 OsCRK5 participates in the response of rice to drought stress A: The expression of OsCRK5 in the leaves after PEG treatment by qPCR detection. B: Phenotype of 20 d rice seedlings treated with 20% PEG. C: Leaf phenotypes of rice seedlings treated with 20% PEG. D: Quantitative PCR to detect the expressions of drought-induced genes in wild-type and OsCRK5-RNAi plants
Fig. 2 Detection of self-activation of plasmid pGBKT7-OsCRK51-444 in yeast cells A: Growth condition of the yeast cells transformed with pGBKT7-OsCRK51-444 plasmid on SD/Trp+X-α-gal plate. B: Growth condition of the yeast cells transformed with pGBKT7-OsCRK51-444 plasmid on single and triple deficiency plates. The growth of yeast transformed with pGBKT7 plasmid was used as control
Fig. 4 Partial positive clones from screened A: Yeast zygote observed under 40× objective lens. B: Partial positive clones screened from SD/-Trp/- Leu/- His/- Ade+X- α-gal+AbA plate
NCBI号 Accession number of NCBI | 基因名称 Gene name | 基因注释 Gene annotation | 参考文献 Reference |
---|---|---|---|
LOC4347054 | ZHD9 | 锌指同源结构域蛋白9 Zinc-finger homeodomain protein 9-like | [ |
LOC107275681 | ARP5 | 肌动蛋白相关蛋白5 Actin-related protein 5 | [ |
LOC4339608 | Di19-1 | 脱水诱导蛋白19 DEHYDRATION-INDUCED 19-like protein | [ |
LOC4336195 | ATP23 | 线粒体内膜蛋白酶 Mitochondrial inner membrane protease | [ |
LOC4329772 | OsFWL2 | 细胞数目调节因子2 Cell number regulator 2 | [ |
LOC4337552 | OseIF3f | 真核翻译起始因子3 Eukaryotic translation initiation factor 3 subunit F | [ |
LOC4329963 | OsCBP20 | 核帽结合蛋白亚基2 Nuclear cap-binding protein subunit 2-like | [ |
LOC4349349 | OsEXPB3 | 类膨胀素B3 Expansin-B3-like | [ |
LOC4334101 | rcs3 | 类半胱氨酸合成酶 Cysteine synthase-like | [ |
LOC4345839 | OsMtATPd2 | 线粒体中d亚单位ATP合成酶 ATP synthase subunit d, mitochondrial | [ |
LOC4325989 | BBTI8 | 胰蛋白酶抑制剂 Bowman-Birk type bran trypsin inhibitor | [ |
LOC4331740 | OsCCR4b | 碳分解代谢抑制蛋白4同源物1 Carbon catabolite repressor protein 4 homolog 1 | [ |
LOC4349455 | BSK2 | 丝氨酸/苏氨酸蛋白激酶 Serine/threonine-protein kinase | [ |
LOC4336306 | OsHCT1 | 羟基肉桂酰转移酶 Hydroxycinnamoyltransferase 1-like | [ |
LOC4330573 | BSH | 染色体结构重塑蛋白 Chromatin structure-remodeling complex protein | |
LOC4340286 | HPGT3 | 羟脯氨酸O-半乳糖基转移酶 Hydroxyproline O-galactosyltransferase | [ |
LOC9269857 | OsWRKY29 | WRKY转录因子29 Probable WRKY transcription factor 29 | [ |
LOC4349610 | OsWRKY70 | WRKY转录因子70 Probable WRKY transcription factor 70 | [ |
LOC4337733 | OsSAMS1 | 类S-腺苷甲硫氨酸合酶1 S-adenosylmethionine synthase 1-like | [ |
LOC4339460 | OsNAP1;2 | 核小体组装蛋白1;2 Nucleosome assembly protein 1;2-like | |
LOC4328653 | OsWR1 | 乙烯应答转录因子WIN1 Ethylene-responsive transcription factor WIN1 | [ |
Table 1 Information of part interacting proteins screened by yeast library
NCBI号 Accession number of NCBI | 基因名称 Gene name | 基因注释 Gene annotation | 参考文献 Reference |
---|---|---|---|
LOC4347054 | ZHD9 | 锌指同源结构域蛋白9 Zinc-finger homeodomain protein 9-like | [ |
LOC107275681 | ARP5 | 肌动蛋白相关蛋白5 Actin-related protein 5 | [ |
LOC4339608 | Di19-1 | 脱水诱导蛋白19 DEHYDRATION-INDUCED 19-like protein | [ |
LOC4336195 | ATP23 | 线粒体内膜蛋白酶 Mitochondrial inner membrane protease | [ |
LOC4329772 | OsFWL2 | 细胞数目调节因子2 Cell number regulator 2 | [ |
LOC4337552 | OseIF3f | 真核翻译起始因子3 Eukaryotic translation initiation factor 3 subunit F | [ |
LOC4329963 | OsCBP20 | 核帽结合蛋白亚基2 Nuclear cap-binding protein subunit 2-like | [ |
LOC4349349 | OsEXPB3 | 类膨胀素B3 Expansin-B3-like | [ |
LOC4334101 | rcs3 | 类半胱氨酸合成酶 Cysteine synthase-like | [ |
LOC4345839 | OsMtATPd2 | 线粒体中d亚单位ATP合成酶 ATP synthase subunit d, mitochondrial | [ |
LOC4325989 | BBTI8 | 胰蛋白酶抑制剂 Bowman-Birk type bran trypsin inhibitor | [ |
LOC4331740 | OsCCR4b | 碳分解代谢抑制蛋白4同源物1 Carbon catabolite repressor protein 4 homolog 1 | [ |
LOC4349455 | BSK2 | 丝氨酸/苏氨酸蛋白激酶 Serine/threonine-protein kinase | [ |
LOC4336306 | OsHCT1 | 羟基肉桂酰转移酶 Hydroxycinnamoyltransferase 1-like | [ |
LOC4330573 | BSH | 染色体结构重塑蛋白 Chromatin structure-remodeling complex protein | |
LOC4340286 | HPGT3 | 羟脯氨酸O-半乳糖基转移酶 Hydroxyproline O-galactosyltransferase | [ |
LOC9269857 | OsWRKY29 | WRKY转录因子29 Probable WRKY transcription factor 29 | [ |
LOC4349610 | OsWRKY70 | WRKY转录因子70 Probable WRKY transcription factor 70 | [ |
LOC4337733 | OsSAMS1 | 类S-腺苷甲硫氨酸合酶1 S-adenosylmethionine synthase 1-like | [ |
LOC4339460 | OsNAP1;2 | 核小体组装蛋白1;2 Nucleosome assembly protein 1;2-like | |
LOC4328653 | OsWR1 | 乙烯应答转录因子WIN1 Ethylene-responsive transcription factor WIN1 | [ |
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