Biotechnology Bulletin ›› 2024, Vol. 40 ›› Issue (1): 332-343.doi: 10.13560/j.cnki.biotech.bull.1985.2023-0492

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Effects of Commensal Microbiota on Intestinal Development, Metabolism, and Mitochondrial Function in Piglets

ZHANG Jin-wei1,2,3(), WU Yuan-xia1,4, SUN Jing1,2,3, LI Xiao-kai1,3,5, LU Lu5, LI Zhou-quan4, GE Liang-peng1,2,3()   

  1. 1. Chongqing Academy of Animal Sciences, Chongqing 402460
    2. Ministry of Agriculture Key Laboratory of Pig Sciences, Chongqing Key Laboratory of Pig Sciences, Chongqing 402460
    3. National Center of Technology Innovation for Pigs, Chongqing 402460
    4. Key Laboratory for Bio-Feed and Molecular Nutrition, College of Animal Science and Technology, Southwest University, Chongqing 400715
    5. College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130
  • Received:2023-05-21 Online:2024-01-26 Published:2024-02-06
  • Contact: GE Liang-peng E-mail:jinweizhang50@163.com;geliangpeng1982@163.com

Abstract:

【Objective】Using pig as animal model to study the regulatory effects of commensal microbiota on host intestinal development and metabolism.【Method】Germ free (GF) piglets and specific pathogen free (SPF) piglets were cultivated through techniques such as aseptic cesarean section and aseptic feeding, and used to study the effects of commensal microbiota on intestinal morphology, metabolism, gene expression and mitochondrial function of piglets through morphological observation, liquid chromatography analysis, RNA seq, and other methods.【Result】Commensal microbiota affected the intestinal morphology and structure, short-chain fatty acid content, amino acid metabolism and mitochondrial content of intestinal cells. Commensal microbiota affected the overall gene expression of the liver, ileum and colon of piglets, and regulated the expression of genes related to biological processes such as mitochondrial oxidation and phosphorylation, amino acid metabolism and lipid metabolism, resulting in the changes in mitochondrial function, thus affected the absorption and metabolism of intestinal nutrients in piglets. 【Conclusion】Commensal microbiota affected the development and metabolism of piglet intestines by regulating the mitochondria of intestinal cells, which provided a theoretical foundation for the study on the regulation of "microbe-host interaction" in piglet’s intestinal health.

Key words: germ-free piglets, specific pathogen-free piglets, commensal microbiota, energy metabolism, mitochondrial function