Research Progress in Increasing Yield of Outer Membrane Vesicles from Gram Negative Bacteria

doi:10.13560/j.cnki.biotech.bull.1985.2023-0540

Abstract

Abstract:

Outer membrane vesicles（OMVs）are vesicle-like structures released by gram-negative bacteria, and OMVs play an important role in gram-negative bacteria growth, infection and communication with the environment. Because OMVs are inactive, non-replicable and possess high immunogenicity, OMVs are widely applied for research and development of novel vaccines. However, the natural OMVs yield is too low to meet scale-up production. To increase OMVs yield is the key technical issue that should be resolved for scale-up production. We mainly review on three aspects for increasing OMVs yield, modification on bacterial genes, optimization on bacterial growth conditions and OMVs production and purification, aiming to provide informative reference for industrial research and development of OMVs vaccine.

Key words: outer membrane vesicles, vaccine development, biogenesis mechanism, yield enhancement mechanism

LIU Can, YAN Xiao-yang, ZENG Yan, OU Xiang-long, LIAO Yong-hong. Research Progress in Increasing Yield of Outer Membrane Vesicles from Gram Negative Bacteria[J]. Biotechnology Bulletin, 2024, 40(3): 100-108.

Figures/Tables 1

References 57

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