Transcriptome and RNA-Seq Technology

Abstract

Abstract: The transcriptome is the complete set of transcripts for certain type of cells or tissues in a specific developmental stage orphysiological condition. Transcriptome analysis can reveal the organism's gene expressing level, structural variation, discovery of new genes. Theresearch methods and platforms of transcriptome are undergoing rapid changes. And bioinformatics analysis is also improved gradually. RNASeqas a new research method, it can be more quickly and afford more accurate transcriptome’s information while using the Next-generationSequencing（NGS）technology. This article compares several main methods and platforms of transcriptome in recent years, and review theprinciple, purpose, steps, bioinformatics’ analysis and applications in related fields of RNA-Seq. This will be afford useful reference for relatedresearch.

Zhang Chunlan, Qin Zijuan, Wang Guizhi, Ji Zhibin, Wang Jianmin. Transcriptome and RNA-Seq Technology[J]. Biotechnology Bulletin, 2012, 0(12): 51-56.

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