Screening and Identification of a Strain Producing L-glutamateOxidase and Its Enzymatic Properties

Biotechnology Bulletin ›› 2012, Vol. 0 ›› Issue (12): 144-149.

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Screening and Identification of a Strain Producing L-glutamateOxidase and Its Enzymatic Properties

Lu Chan, Zheng Pu, Sun Zhihao

The Key Laboratory of Industrial Biotechnology，Ministry of Education，School of Biotechnology，Jiangnan University，Wuxi 214122

Received:2012-06-04 Revised:2013-01-25 Online:2012-12-26 Published:2013-02-06

Abstract

Abstract: Chromogenic 4-Aminoantipyrine/phenol reaction was used in the procedure of strain screening. A strain named Str-6 wasobtained. Taxonomy of the strain, based on its morphological, physiological and biochemical characteristics, as well as the sequence of 16SrDNA, was classified to Streptomyces collinus. The preliminary fermentation test showed that strain Str-6 could produced extracellular L-glutamateoxidase, and the maximal yield could reached 0.023 1 U/mg protein cultured at 28℃ for 36 h. It has strong substrate specificity for L-glutamate.The optimal pH was 6.0 and the temperature of the L-glutamate oxidase was 35℃ . The enzyme exhibited high thermal stability at 35-40℃ andstable at pH6.0--7.0.

Lu Chan, Zheng Pu, Sun Zhihao . Screening and Identification of a Strain Producing L-glutamateOxidase and Its Enzymatic Properties[J]. Biotechnology Bulletin, 2012, 0(12): 144-149.

References

[1] 毕春元, 李玲, 李敬龙. L-谷氨酸氧化酶的研究进展. 生命科学,2012, 24(2)：169-173.
[2] 郑强, 严卫民, 徐东, 等. L-谷氨酸氧化酶的来源和特性及其在生化检验中的应用. 检验医学教育, 2003, 10(2)：32-35.
[3] Kamei T, Asano K, Suzuki H, et al. L-glutamate oxidase from Streptomycesviolascens.Ⅰ. Production, isolation and some properties. Chemicaland Pharmaceutical Bulletin, 1983, 31(4)：1307-1314.
[4] Kusakabe H, Midorikawa Y, Fujishima T, et al. Purification and propertiesof a new enzyme L-glutamate oxidase, from Streptomyces sp.X-119-6 grown on wheat bran. Agricultural and Biological Chemistry,1983, 47(6)：1323-1328.
[5] Bǒhmer A, Müller A, Passarge M, et al. A novel L-glutamate oxidasefrom Streptomyces endus purification and properties. European Journalof Biochemistry, 1989, 182(2)：327-332.
[6] Sukhacheva MV, Netrusov AI. Extraeellular L-glutamate oxidase ofStreptomyces sp. Z-11-6: obtainment and properties. Microbiology,2000, 69(1)：17-20.
[7] Chen CY, Wu WT, Huang CJ, et al. A common precursor for thethree subunits of L-glutamate oxidase encoded by gox gene fromStreptomyces platensis NTU3304. Canadian Journal of Microbiology,2001, 47(3)：269-275.
[8] Arima S, Sasaki C, Sakaguchi C, et al. Structural characterization ofL-glutamate oxidase from Streptomyces sp. X-119-6. FEBS Journal,2009, 276(14)：3894-3903.
[9] 徐水清, 李友荣. L-谷氨酸氧化酶产生菌的筛选与产酶条件.微生物学报, 1993, 33(4)：309-312.
[10] 李青山, 李友荣, 闵简书. L-谷氨酸氧化酶高产菌株的快速筛选及诱变育种. 华东化工学院学报, 1993, 19(2)：148-153.
[11] 沈群. 链霉菌L-谷氨酸氧化酶发酵条件的研究 . 中国机械工程学会包装与食品工程分会第四届学术年会论文集
[C] .1993: 64-70.
[12] 郑雅楠, 杨宇, 吕国忠, 等. 土壤放线菌分离方法研究. 安徽农业科学, 2006, 34(6)：1167-1170.
[13] Bradford MM. A ripid and sensitive method for the quantitation ofmicrogram quantities of protein utilizing the principle of proteindyebinding. Analytical Biochemistry, 1976, 72: 248-254.
[14] 中国科学院微生物研究所放线菌分类组. 链霉菌鉴定手册[M] . 北京：科学出版社, 1975: 14-16.
[15] 任增亮, 张东旭, 俞梅英, 等. Streptomyces hygroscopicus 谷氨酰胺转胺酶酶原基因的鉴定及在大肠杆菌中的表达. 微生物学报, 2008, 48(4)：480-485.
[16] 东秀珠, 蔡妙英. 常见细菌系统鉴定手册[M] . 北京：科学出版社, 2001: 349-399.

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