Recent Advances in ANL Andenylating Enzymes

Abstract

Abstract: The ANL adenylating enzymes contains acyl- and aryl-CoA synthetases, firefly luciferase, and the adenylation domains of the modular non-ribosomal peptide synthetases（NRPSs）. Members of this family catalyze a two-step reaction ：the initial adenylation of a carboxylate to form an acyl-AMP intermediate, the second step commonly for formation of a thioester. With more members of the adenylateforming family of enzymes being identified, 10 conserved and important sequence motifs for catalytic activity have been revealed. The comprehensive studies of the structures and kinetics provide insight into the role of the rotation of the C-terminal sub-domain in the catalytic cycle. Such a rotation, a common strategy used by ANL adenylating enzymes, can present different conformation of the active sites to catalyze different partial reactions. In this paper，we describe the three types of ANL adenylating enzymes and summarize the research course of the ANL superfamily enzymes，conserved regions and conformational dynamics of enzymes in the process of the enzymatic reactions.

Key words: Adenylating enyzmes, Acyl-CoA synthetases, Firefly luciferase, Adenylation domain, Conformational dynamics

Li Lili, Lin Shuangjun. Recent Advances in ANL Andenylating Enzymes[J]. Biotechnology Bulletin, 2013, 0(4): 14-20.

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