Cloning and Sequence Analysis of 5&apos;-Flanking Region of Bovine FATP1 Gene

Abstract

Abstract: The experiment was conducted to clone 5'-flanking region of bovine FATP1 gene to study regulatory mechanism of the specific transcriptional regulation region. The 5'-flanking region of bovine FATP1 gene was amplified with PCR, and then the 2 164 bp (-1969- +194 bp) 5'-flanking promoter sequence was obtained by product purification, ligation, transformation, and sequence comparison. The obtained cloning vector of 5'-flanking region of bovine FATP1 gene was analyzed by the promoter software in this study. The results indicated that there were 4 transcription start sites and 40 potential transcription factor binding sites. Comparing bovine with human, mouse, rat and dog, the homology of 5'-flanking promoter sequence were 74.1%, 71.1%, 72.0%, and 62.8%, respectively. The conservative region was mainly concentrated in -578- -93 bp of the upstream of transcription start sites, and the highly conserved region was in -265- -162 bp of the upstream of transcription start sites, which may be conclude that the -265- -162 bp of the upstream of transcription start sites was the core promoter region of the gene. The 2 164 bp 5'-flanking region of the bovine FATP1 gene was cloned successfully and the core promoter region was preliminary predicted in the gene.

Key words: Bovine, Cloning FATP1 gene, Promoter, Transcription factor

Gong Ting, Xu Houqiang, Chen Wei, Zhao Jiafu, Luo Heng, Chen Xiang, Zhang Yong. Cloning and Sequence Analysis of 5'-Flanking Region of Bovine FATP1 Gene[J]. Biotechnology Bulletin, 2013, 0(5): 86-92.

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Cloning and Sequence Analysis of 5'-Flanking Region of Bovine FATP1 Gene

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