Biotechnology Bulletin ›› 2013, Vol. 0 ›› Issue (6): 104-109.

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Study on the SNP and Bioinformatics of Promoter Region of JAK2 Gene in Cattle

Gong Yu1 Yang Yongqiang2 Jiao Rengang1 Hui Yanting2 Liu Ruoyu2   

  1. (1. Animal Science and Technology Station of Guizhou, Guiyang 550001;2. College of Animal Sciences, Guizhou University, Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, Guizhou Key Laboratory of Animal Genetics, Breeding and Reproduction, Guiyang 550025)
  • Received:2013-06-20 Revised:2013-06-20 Online:2013-06-20 Published:2013-06-20

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Abstract: In order to screen the polymorphisms of JAK2 promoter in cattle and analyze the effect of SNPs on function elements of promoter. Two cattle breeds(Wuchuan black cattle and Guizhou Holstein cow)with significant difference in breeds property were selected to construct DNA pools, SNP sites were screened by direct sequencing subsequently. Results showed that 2 single nucleotide polymorphisms(SNPs)were found in the 5' flanking region and part of exon 1 which included G+5A and G+104A. Furthermore, bioinformatics tools were used to predict the core region of the promoter. It demonstrated that 1 new transcription factors binding sites emerged while 9 previous transcription factors binding sites disappeared based on the SNPs found in this study, and it also showed that SNP of G+5A could change the secondary structure of RNA, transcription factors binding sites and range of CpG island dramatically by using various softwares.

Key words: JAK2, SNP, Promoter, Guizhou Holstein cow, Wuchuan black cattle