Abstract: The genetic relationship of Conyza blinii Lévl. was analyzed in molecular level and the distribution characteristics of total flavonoid constituents. The RAPD method was applied to the study on Conyza blinii Lévl. from different germplasms by use of 10 random primers about 10 bp, and the parameters were calculated by NTSYSpc2.1 and the relationship was constructed based on UPGMA. Use UV spectrophotometry to test total flavonoids in each sample. 10 primers screened out from 100 primers were used for RAPD amplification. A total of 71 bands were generated, of which 68 bands were polymorphism bands. According to RAPD analysis, 18 germplasms of Conyza blinii Lévl. were classified into two categories. The genetic diversity of the 18 species in Conyza blinii Lévl. is high and the total flavinoids was related of geographic distribution.Key words: Conyza blinii Lévl. RAPD Cluster analysis Total flavonoids