Abstract: Spermatids are essential cells in testes of male animals during spermatogenesis. The rsb66 gene has been reported expressing in spermatids specifically. However, the DNA sequence of rsb66 gene in Cashmere goat have not been reported. In this study, we designed primers according to the reported rsb66 gene cDNA sequences from other species in the GenBank. The coding sequence was amplified by RT-PCR. The PCR fragment was inserted into the T-A cloning vector pMD19-T. The sequencing result showed that the coding sequence of Cashmere goat rsb66 gene possesses 97.0% homology compared with that of bos Taurus. Such highly homology between Cashmere goat and Bos taurus suggests that this gene is conserved in the evolution.