Abstract: In order to investigate the cytochrome P450 CYP6B6 in midgut induced by flavones and terpenes extracts from Artemisia rupestris, we detected the CYP6B6 expression in mRNA level by Real-time quantitative PCR（qPCR）, and at protein level by Western blot.qPCR results showed that the P450 CYP6B6 mRNA levels in the midgut of the larvae were induced by these two extracts. For the time effect, when the larvae were exposed to 20 mg/100 g flavones extracts and 2 mg/100 g terpenes extracts respectively for 12 h the relative expression level of P450 CYP6B6 mRNA in the midgut was over-expressed, while at 24 h the expression was inhibited. For the concentration effect, when the larvae were treated with these two exyracts for 24 h, the relative expression level of P450 CYP6B6 mRNA was over-expressed by 20 mg/100 g flavones and 1 mg/100 g terpenes extracts, respectively, while the expression was inhibited by 40 mg/100 g flavones and 2 mg/100 g terpenes extrcts in the diet, respectively.