Algae-bacteria Symbiosis Increases Biomass and Oil Production of Chlorella emersonii

doi:10.13560/j.cnki.biotech.bull.1985.2018-1098

Abstract

Abstract: The large-scale cultivation of microalgae is often accompanied by bacteria and their infection. Limited information is available for the effect of bacteria in phycosphere on microalgae growth and the mechanism of algae-bacterial symbiosis. In order to establish a beneficial algae-bacterial symbiotic system and increase the biomass production of microalgae,Chlorella emersonii was selected as the test material,and microflora in phycosphere was isolated and then identified by 16S rDNA sequencing. The dominant growth-promoting bacteria were screened by co-culture of algae and bacteria（1∶1）. Various algae-bacterial co-culture systems at different ratios were artificially constructed to analyze the effects of dominant growth-promoting bacteria on microalgae growth and biomass yield. The results showed that 6 bacteria were isolated from the phycosphere of C. emersonii strain SXND-25,belonging to the 4 genera Pantoea,Pseudomonas,Enterococcus gallinarum and Escherichia coli. Of them,Pseudomonas and Pantoea were the dominant growth-promoting bacteria. Compared with other co-culture systems at different ratios of algae and bacteria,a symbiotic system of C. emersonii and Pantoea（1∶5）showed outstanding growth-promoting effects. The biomass of C. emersonii increased to 5.86 g/L on the 8th day of cultivation,the oil content was 26.88% in algal cells,with 1.575 g/L total oil production and 554-564 mg/L monounsaturated fatty acid（MUFA）. Another excellent combination was 1∶1 co-culture of C. emersonii and Pseudomonas. The biomass of C. emersonii on the 8th day was 4.12 g/L,the oil content of algal cells was 29.50%,and the total oil production reached 1.215 g/L,but low MUFA content（168-175 mg/L）. The present study demonstrated that adding the appropriate amount of growth-promoting bacteria in the cultivation of C. emersonii simultaneously increased algal biomass and oil production,providing solid scientific reference for investigating the algal-bacterial interaction effect and the application of beneficial algal-bacterial symbiosis system in commercial production of microalgae.

Key words: Chlorella emersonii, bacterial flora/community in phycosphere, algae-bacteria symbiosis, algal biomass, oil accumulation.

ZHAGN Jing-jie, DUAN Lu-lu, CHENG Wei-lan, JI Chun-li, CUI Hong-li, LI Run-zhi. Algae-bacteria Symbiosis Increases Biomass and Oil Production of Chlorella emersonii[J]. Biotechnology Bulletin, 2019, 35(5): 76-84.

References

[1] Abomohra EF, Jin W, Tu R, et al.Microalgal biomass production as a sustainable feedstock for biodiesel:Current status and perspectives[J]. Renewable and Sustainable Energy Reviews, 2016, 64(1):596-606.
[2] 黄英明, 王伟良, 李元广, 等.微藻能源技术开发和产业化的发展思路与策略[J].生物工程学报, 2010, 26(7):907-913.
[3] 李润植, 季春丽, 崔红利.微藻生物技术助力功能农业[J].山西农业大学学报:自然科学版, 2018, 38(3):1-12+77.
[4] 郑洪立, 张齐, 马小琛, 等.产生物柴油微藻培养研究进展[J].中国生物工程杂志, 2009, 29(3):110-116
[5] Reddy MS, Md. YF, Sanjoy B, et al. Microalgae as Sustainable Renewable Energy Feedstock for Biofuel Production[J]. BioMed Research International, 2015, 2015(9):1-13.
[6] 王玉荣, 师文静, 佟明友, 等.产油微藻的分离筛选与鉴定[J].食品与生物技术学报, 2016, 35(1):77-81.
[7] 史飞飞, 陈通, 程蔚兰, 等.酸驯化和紫外诱导提高微藻耐酸性[J].生物技术通报, 2017, 33(8):146-151.
[8] 刁梦洁, 柳杰, 王晚晴, 等.菌藻共生对污水处理和微藻生物量积累的影响[J].环境工程, 2018, 36(3):8-12.
[9] 王荣昌, 程霞, 曾旭.污水处理中菌藻共生系统去除污染物机理及其应用进展[J].环境科学学报, 2018, 38(1):13-22.
[10] 尚海, 薛林贵, 马萍, 等.小球藻藻菌共生体系在产油方面的特性[J].微生物学通报, 2017, 44(10):2280-2288.
[11] 马浩天, 李润植, 张宏江, 等. 基于微藻培养处理畜禽养殖废水的研究进展[J].生物技术通报, 2017, 10(9):1-8.
[12] 程蔚兰, 邵雪梅, 宋程飞, 等.氮胁迫对埃氏小球藻生长及油脂积累的影响[J].生物技术通报, 2017, 33(11):160-165.
[13] 张飞, 高秀清, 张靖洁, 等.种子特异表达异源DGAT1基因提高大豆种子含油量和营养品质[J].生物工程学报, 2018, 34(9):1478-1490.
[14] Amin SA, Green DH, Hart MC, et al.Photolysis of iron-siderophore chelates promotes bacterial-algal mutualism[J]. Proceedings of the National Academy of Sciences, 2009, 106(40):17071-17076.
[15] 张晶, 侯和胜, 佟少明. 微藻与细菌作用关系的研究进展[J]. 激光生物学报, 2016, 25(5):385-390+417.
[16] 杨世平, 刘慧玲, 黄翔鹄, 等. 一株促藻生长细菌培养条件的优化[J]. 广东海洋大学学报, 2010, 30(6):21-24.
[17] Xi Yang, Ping Xie, Yunzhen Yu, et al.Microcystis aeruginosa/Pseudomonas pseudoalcaligenes interaction effects on off-flavors in algae/bacteria co-culture system under different temperatures[J]. Journal of Environmental Sciences, 2015, 31(5):38-43.
[18] 涂仁杰, 金文标, 韩松芳, 陈洪一. 细菌对城市污水中小球藻生长和油脂积累的影响[J]. 环境科学, 2017, 38(10):4279-4285.
[19] Suminto, Kazutsugu Hirayama.Application of a growth-promoting bacteria for stable mass culture of three marine microalgae[J]. Hydrobiologia, 1997, 358(1/3):223-230.
[20] 史玉倩, 赵艳. 水稻种子内生泛菌促进小球藻生长和油脂积累[J]. 中国农业科学, 2016, 49(8):1429-1442.
[21] Mouget JL, Dakhama A, Lavoie MC, et al.Algal growth enhancement of bacteria:is consumption of photosynthetic oxygen involved?[J]. Fems Microbiology Ecology, 2010, 18(1):3-5.
[22] 章登岚, 赵以军, 吴刚, 等. 不同来源溶藻菌的分离、鉴定及溶藻效果比较[J]. 微生物学杂志, 2017, 37(3):100-104.
[23] Abed RM.Interaction between cyanobacteria and aerobic heterotrophic bacteria in the degradation of hydrocarbons[J]. International Biodeterioration & Biodegradation, 2010, 64(1):58-64.
[24] 冯洁. 植物病原细菌分类最新进展[J]. 中国农业科学, 2017, 50(12):2305-2314.
[25] 曹慧英, 李洪杰, 朱振东, 等. 玉米细菌干茎腐病菌成团泛菌的种子传播[J]. 植物保护学报, 2011, 38(1):31-36.
[26] 顾沁, 张昊, 黄海, 等. 一种玉米新型细菌性褐腐病的病原鉴定[J]. 植物保护, 2016, 42(3):87-90.
[27] 厉艳, 王英超, 宋涛, 等. 进境多肉植物细菌性褐腐病的病原分离与鉴定[J]. 食品安全质量检测学报, 2017, 8(11):4143-4146.
[28] 涂泽敏, 吴芳燕, 罗剑飞, 等. 产油脂微藻的分离、鉴定及筛选[J]. 现代食品科技, 2018, 10(5):1-6.