Inheritance Study on Fruiting Body Color of Pleurotus djamor（Rumph. ex Fr.）Boedijn

doi:10.13560/j.cnki.biotech.bull.1985.2019-0127

Abstract

Abstract: The color of fruiting body is one of the most important commercial traits of edible mushrooms and an important objective for breeding superior mushroom cultivars. In this study,a pink strain CCMSSC00450 and a taupe strain CCMSSC04445 of Pleurotus djamor（Rumph. ex Fr.）Boedijn were used as dikaryotic parental lines,and the constitutive monokaryons of two strains i.e.,PDm52 and PDm17 for strain CCMSSC00450,PDm99 and PDm14 for CCMSSC04445,were obtained by protoplast monokaryonization. After the cross-mating of 4 constitutive monokaryons,4 hybrid dikaryons were generated and cultivated for phenotyping. The 4 hybrid strains showed different color,i.e.,pink for PDm52×PDm99,pinkish gray for PDm52×PDm14,taupe for PDm17×PDm14,and white for PDm17×PDm99. A total 207 single spore isolates of the pink hybrid（PDm52×PDm99）were randomly selected as the segregating population of the color of pink fruiting body and PDm17 as the tester line,the test-cross population was constructed. Fruiting results showed that the color of the test-cross population was obviously segregated in a continuous distribution pattern varying from dark to light with pink,light pink,white,etc. The results revealed that the pink of the fruiting body was not completely dominant with respect to the white,and the white was recessive. Color of fruiting body of P. djamor is a quantitative trait controlled by multiple genes with high heritability. Meanwhile,a significant correlation was detected between the colors of the cap and gill.

Key words: Pleurotus djamor, color of the fruiting body, quantitative trait, test-cross, hybridization

ZHANG Ze-hua, GAO Wei, WU Xiang-li, HUANG Chen-yang, CHANG Ming-chang, ZHANG Jin-xia. Inheritance Study on Fruiting Body Color of Pleurotus djamor（Rumph. ex Fr.）Boedijn[J]. Biotechnology Bulletin, 2019, 35(5): 70-75.

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