大蒜素及其前药对食管癌细胞生长的影响

doi:10.13560/j.cnki.biotech.bull.1985.2015.02.034

生物技术通报 ›› 2015, Vol. 31 ›› Issue (2): 228-232.doi: 10.13560/j.cnki.biotech.bull.1985.2015.02.034

• 研究报告 • 上一篇

大蒜素及其前药对食管癌细胞生长的影响

杨金部¹, 常全娥¹, 苟萍¹, 魏鸿雁²

1.新疆大学生命科学与技术学院，乌鲁木齐 830046;
2.新疆维吾尔自治区中药民族药研究所，乌鲁木齐 830002

收稿日期:2014-06-27 出版日期:2015-02-05 发布日期:2015-02-06
作者简介:杨金部，女，硕士研究生，研究方向：食品工程；E-mail：yangjinbu2010@126.com
基金资助:
新疆自治区高校科研计划（XJEDU02010I13）

Growth Influence of Allicin and Its Prodrug on Esophageal Cancer Cells

Yang Jinbu¹, Chang Quan'e¹, Gou Ping¹, Wei Hongyan²

1. College of Life Science and Technology, Xinjiang University, Urumqi 830046;
2. Xinjiang Institute of Chinese Medicine and Medicine of National Minorities, Urumqi 830002

Received:2014-06-27 Published:2015-02-05 Online:2015-02-06

摘要/Abstract

摘要： 通过大蒜素及大蒜素前药处理食管癌Eca9706细胞，观察细胞形态，测定乳酸脱氢酶（LDH）活性和DNA降解。结果表明，癌细胞变圆、变小，活细胞数目减少，脱壁细胞和细胞碎片增多。DNA-Ladder试验结果显示，出现了DNA梯状条带，表明DNA分子发生降解。大蒜素及其前药处理的Eca9706细胞LDH活性显著高于对照组，随处理浓度增加和时间延长，LDH活性依次升高，表现出时间和剂量的依赖性。大蒜素前药处理Eca9706细胞的LDH活性明显高于大蒜素，表明大蒜素前药对细胞膜的损伤程度较大。

关键词: 大蒜素, 大蒜素前药, 食管癌, 细胞形态, DNA降解

Abstract: We observed the cancer cell morphology, determined lactate dehydrogenase（LDH） activity and DNA degradation after allicin and its prodrug（alliin + alliinase） treated human esophageal cancer Eca9706 cells. The results showed that the cancer cells became more circular and smaller, the number of living cells decreased, the non-adherent cells and cell debris increased. DNA-Ladder revealed to exist DNA ladder line, which indicated DNA molecule degraded. Cancer cells’ LDH activity treated with allicin and its prodrug was significantly higher than the control group. LDH activity had a time and dose dependent manner with treated concentration increased and time extended. Eca9706 cells’ LDH activity by the prodrug treated was higher than allicin, this confirmed prodrug could cause great damage on cell membrane.

Key words: allicin, allicin prodrug, human esophageal cancer, cell morphology, DNA degradation

杨金部, 常全娥, 苟萍, 魏鸿雁. 大蒜素及其前药对食管癌细胞生长的影响[J]. 生物技术通报, 2015, 31(2): 228-232.

Yang Jinbu, Chang Quan'e, Gou Ping, Wei Hongyan. Growth Influence of Allicin and Its Prodrug on Esophageal Cancer Cells[J]. Biotechnology Bulletin, 2015, 31(2): 228-232.

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大蒜素及其前药对食管癌细胞生长的影响

Growth Influence of Allicin and Its Prodrug on Esophageal Cancer Cells

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