生物技术通报 ›› 2015, Vol. 31 ›› Issue (3): 43-48.doi: 10.13560/j.cnki.biotech.bull.1985.2015.04.006

• 技术与方法 • 上一篇    下一篇

SSR分子标记在烟草研究中的应用进展

陈杰1, 杨静1, 龙胜贤1, 肖慈平1, 杨昌义1, 黄清忠1, 王维2   

  1. (1.贵州省黔东南州烟草公司,凯里 556000;2. 华南农业大学农学院 华南农业大学烟草研究室,广州 510642)
  • 收稿日期:2014-07-30 出版日期:2015-03-16 发布日期:2015-03-16
  • 作者简介:陈杰,男,硕士研究生,研究方向:烟叶生产及科技推广应用;E-mail:hnchenjie002@126.com
  • 基金资助:
    国家烟草专卖局重点项目(110200902043)

Advance of the ApplicationofSSRMolecularMarkersinTobaccoResearch

Chen Jie1 Yang Jing2 Long Shengxian1 Xiao Ciping1 Yang Changyi1 Huang Qingzhong1 Wang Wei2   

  1. (1. Qiandongnan Tobacco Company in Guizhou Province,Kaili 556000;2. Tobacco Laboratory,College of Agriculture,South China Agricultural University,Guangzhou 510642)
  • Received:2014-07-30 Published:2015-03-16 Online:2015-03-16

摘要: SSR分子标记技术作为最常用的分子标记技术之一,该标记技术重复性好,结果可靠,近年来在烟草遗传育种中展示了广阔的应用前景,是应用潜力较大的分子标记技术。介绍了SSR分子标记的原理及其分布特征,对其在烟草基因定位及分子标记辅助选择、种质资源研究、遗传图谱构建及种子纯度及真伪鉴定研究中的应用等方面进行了综述,并探讨了SSR分子标记技术在烟草遗传育种中的应用前景,以期为烟草SSR分子标记技术的研究提供参考。

关键词: 烟草, 分子标记, SSR, 遗传育种

Abstract: SSR molecular marker technology as one of the most commonly used molecular markers, the marker technique result is reliable and good repeatability. In recent years, SSR marker shows a broad application prospect in tobacco genetic breeding, is a large potential for application of molecular markers. This paper introduced the principle and distribution characteristics of SSR molecular marker, and summarized the study of gene location and molecular marker-assisted selection, germplasm research, the construction of molecular genetic maps and the seed purity identification and authenticity of tobacco, and analysed the application prospect of molecular markers in tobacco genetic breeding. The main aim is to supply useful information so as to promote the development of SSR molecular marker technology research in tobacco.

Key words: tobacco, molecular marker, SSR, genetic breeding