生物技术通报 ›› 2015, Vol. 31 ›› Issue (8): 193-199.doi: 10.13560/j.cnki.biotech.bull.1985.2015.08.028

• 研究报告 • 上一篇    下一篇

猪胰脏羧肽酶转酯功能和耐酸抗蛋白酶特性研究

冯士元, 孙同韦, 牟慧艳, 张会图, 路福平   

  1. 天津市工业微生物重点实验室 工业发酵微生物教育部重点实验室 天津科技大学生物工程学院,天津 300457
  • 收稿日期:2015-03-20 出版日期:2015-08-21 发布日期:2015-08-22
  • 作者简介:冯士元,硕士研究生,研究方向:微生物与生化药学;E-mail:tianjinfsy123@163.com
  • 基金资助:
    国家“863”计划项目(2013AA102803)

A Study on Transesterification and Acid-resistant Antiprotease of Carboxypeptidase in Porcine Pancreas

Feng Shiyuan, Sun Tongwei, Mou Huiyan, Zhang Huitu, Lu Fuping   

  1. Tianjinn Key Laboratory of Industrial Microbiology,Key Laboratory of Industrial Fermentation Microbiology Ministry of Education,College of Biotechnology,Tianjin University of Science&Technology,Tianjin 300457
  • Received:2015-03-20 Published:2015-08-21 Online:2015-08-22

摘要: 羧肽酶(carboxypeptidase)是一类可水解肽链C末端氨基酸残基的蛋白酶,可用作饲料添加剂,促进畜禽生长。猪胰脏中含有一种羧肽酶A1(CPA1),该酶除能水解蛋白质外,还能分解油脂。CPA1在以酪蛋白和橄榄油为底物时,比活力分别为53.14 U/mg和22.4 U/mg;经过pH2.0酸性环境和胰蛋白酶处理后,其酶活残留率分别为95.65%和78.14%。结果表明,CPA1具有转酯功能和耐酸抗蛋白酶特性。

关键词: 羧肽酶A1, 转酯功能, 耐酸抗蛋白酶

Abstract: Carboxypeptidase is a kind of protease that cleaves the peptide bond of an amino acid residue at the C-terminal end. It can be used in feed additives, and promotes the growth of livestock and poultry. A carboxypeptidase A1(CPA1)was obtained from porcine pancreas, and it can hydrolyze proteins and decompose oils. It showed the specific activity as 53.14 U/mg and 22.4 U/mg when casein and olive oil used as substrate, respectively. The CPA1’s relative activity reached 95.65% and 78.14% respectively after the treatment of pH2.0 acidic and trypsin. The results indicate that the CPA1 has the function of transesterification and the feature of distinct acid-resistant antiprotease.

Key words: carboxypeptidase A1, function of transesterification, acid-resistant antiprotease