东北林蛙发育早期TLR2的表达及免疫应答特征

doi:10.13560/j.cnki.biotech.bull.1985.2015.10.001

生物技术通报 ›› 2015, Vol. 31 ›› Issue (10): 230-235.doi: 10.13560/j.cnki.biotech.bull.1985.2015.10.001

东北林蛙发育早期TLR2的表达及免疫应答特征

王泽¹,赫天一²,陆宇燕¹

1.沈阳师范大学化学与生命科学学院, 沈阳 110034;
2.沈阳医学院附属中心医院中心实验室, 沈阳 110024

收稿日期:2015-01-23 出版日期:2015-10-28 发布日期:2015-10-28
作者简介:王泽, 女, 博士, 研究方向：发育免疫学; E-mail：synu.wangze@aliyun.com
基金资助:
沈阳师范大学博士启动基金项目（054-55440107029）, 沈阳师范大学生态与环境研究中心主任基金项目（EERC-Y-201404）

The Expression and Immune Response Characteristics of TLR2 at the Early Development Stage of Rana chensinensis

Wang Ze, He Tianyi, Lu Yuyan

College of Chemistry and Life Science, Shenyang Normal University, Shenyang 110034; Central Hospital Affiliated To Shenyang Medical College, Shenyang 110024

Received:2015-01-23 Published:2015-10-28 Online:2015-10-28

摘要/Abstract

摘要： 旨在研究东北林蛙发育早期TLR2的表达特征及其针对不同PAMPs的免疫应答特征。免疫组化染色及RT-PCR对11期至42期东北林蛙TLR2进行定位和定量检测; 29期蝌蚪0.5 μg/mL的LTA, LPS, ZymoA, Poly I:C急性腹腔注射, RT-PCR检测24 h内TLR2 mRNA相对表达量变化。结果显示, TLR2在东北林蛙发育早期的组织中分布广泛, 11期TLR2 mRNA相对表达量0.187±0.021, 25期升至0.373±0.031后, 42期降至0.170±0.020。24 h内未检测到LPS组和Zymo A组TLR2 mRNA相对表达量显著性变化（P>0.05）, LTA组在4 h与12 h出现两次极显著性升高（P<0.01）; PolyI:C组于8 h至峰值（P<0.01）。东北林蛙发育早期TLR2的时空表达特征与其发育阶段的生境变化相适应, 29期东北林蛙的TLR2对4种PAMPs识别和应答兼具了鱼类和哺乳动物的特征, 体现了免疫系统在进化上的演变。

关键词: 东北林蛙, Toll样受体2, 免疫应答, 病原相关分子模式

Abstract: It was to study the expression and immune response characteristics of TLR2 at the early development stage of Rana chensinensis. Immunohistochemistry and RT-PCR were used to locate and quantify the TLR2 mRNA from stage 11 to stage 42 of Rana chensinensis. Tadpoles at stage 29 were injected with antigen LTA, LPS, ZymoA and Poly I:C intraperitoneally. The expression changes of TLR2 mRNA were detected by RT-PCR within 24 h. TLR2 is widely distributed at the early development stage of Rana chensinensis. The relative expression of TLR2 mRNA increased form 0.187±0.021 to 0.373±0.031 at stage 25, and then returned to 0.170±0.020 progressively. The expression of TLR2 mRNA had no significant change in the LPS group and the Zymo A group（P>0.05）. TLR2 mRNA of the LTE group peaked twice at 4 h与12 h after antigen injection（P<0.01）; and the PolyI：C group peaked at 8 h（P<0.01）. The expression character of TLR2 matches with its living environment at the early development stage of Rana chensinensis.TLR2 did not responses to the challenge of LPS and ZymoA timely at the stage 29, on the other hand, TLR2 recognized and responsed to LTA and PolyI:C group effectively. TLR2 of Rana chensinensis possesses the recognition and response characteristics to PAMPs both of fish and mammals.

Key words: Rana chensinensis, TLR2, immune response, pathogen-associated molecular patterns

王泽,赫天一,陆宇燕. 东北林蛙发育早期TLR2的表达及免疫应答特征[J]. 生物技术通报, 2015, 31(10): 230-235.

Wang Ze, He Tianyi, Lu Yuyan. The Expression and Immune Response Characteristics of TLR2 at the Early Development Stage of Rana chensinensis[J]. Biotechnology Bulletin, 2015, 31(10): 230-235.

参考文献

[1] Lester SN, Li K. Toll-like receptors in antiviral innate immunity[J]. J Mol Biol, 2014, 426（6）：1246-1264.
[2] Satake H, Sekiguchi T. Toll-like receptors of deuterostome inverteb-rates[J]. Front Immunol, 2012, 3：34-40.
[3] Quiniou SM, Boudinot P, Bengtén E. Comprehensive survey and genomic characterization of Toll-like receptors（TLRs）in channel catfish, Ictalurus punctatus：identification of novel fish TLRs[J]. Immunogenetics, 2013, 65（7）：511-530.
[4] 孙红娟, 周遵春, 崔军, 等. 海洋动物Toll样受体的研究进展[J]. 生物技术通报, 2013（1）：41-48.
[5] Ramasamy KT, Verma P, Reddy MR. Toll-like receptors gene expression in the gastrointestinal tract of Salmonella serovar Pullorum-infected broiler chicken[J]. Appl Biochem Biotechnol, 2014, 173（2）：356-364.
[6] Roy R, Kumar D, Sharma A, et al. ZnO nanoparticles induced adjuvant effect via toll-like receptors and Src signaling in Balb/c mice[J]. Toxicol Lett, 2014, 230（3）：421-433.
[7]Klawitter M, Hakozaki M, Kobayashi H, et al. Expression and regulation of toll-like receptors（TLRs）in human intervertebral disc cells[J]. Eur Spine J, 2014, 23（9）：1878-1891.
[8]Ishii A, Kawasaki M, Matsumoto M, et al. Phylogenetic and expression analysis of amphibian Xenopus Toll-like receptors[J]. Immunogenetics, 2007, 59（4）：281-293.
[9]吴立舒. 彩虹病毒胁迫下东北林蛙皮肤TLR mRNA的差异表达[D]. 哈尔滨：东北林业大学, 2012.
[10] 王寿兵, 张思路, 屈云芳, 等. 辽宁产中国林蛙早期胚胎发育研究[J]. 复旦学报：自然科学版, 1996, 35（2）：163-169.
[11] Abdi J, Garssen J, Redegeld F. Toll-like receptors in human multiple myeloma：new insight into inflammation-related pathogenesis[J]. Curr Mol Med, 2014, 14（4）：423-431.
[12] Du Pasquier L, Robert J, et al. B-cell development in the amphibian Xenopus[J]. Immunological Reviews, 2000, 175：201-213.
[13]Samanta M, Swain B, et al. Molecular characterization of toll-like receptor 2（TLR2）, analysis of its inductive expression and asso-ciated down-stream signaling molecules following ligands exposure and bacterial infection in the Indian major carp, rohu（Labeo rohita）[J]. Fish Shellfish Immunol, 2012, 32（3）：411-425.
[14]韦友传. 斜带石斑鱼TLR1/TLR2和MyD88基因的克隆与免疫应答研究[D]. 南宁：广西大学, 2011.
[15]Baoprasertkul P, Peatman E, et al. Structural characterisation and expression analysis of toll-like receptor 2 gene from catfish[J]. Fish Shellfish Immunol, 2007, 22（4）：418-426.
[16] Park OJ, Han JY, et al. Lipoteichoic acid of Enterococcus faecalis induces the expression of chemokines via TLR2 and PAFR signal-ing pathways[J]. J Leukoc Biol, 2013, 94（6）：1275-1284.
[17]Oliveira-Nascimento L, Massari P, Wetzler LM. The role of TLR2 in infection and immunity[J]. Front Immunol, 2012, 3：79-95.
[18]战仁慧. TLR2, TLR4和TLR9在小鼠抵抗鼠伤寒沙门氏菌感染中的作用[D]. 济南：山东大学, 2013.
[19]Wypasek E, Natorska J, et al. Toll-like receptors expression and NF-κB activation in peritoneal leukocytes in morphine-mediated impairment of zymosan-induced peritonitis in swiss mice[J]. Arch Immunol Ther Exp（Warsz）, 2012, 60（5）：373-382.
[20]Facci L, Barbierato M, Marinelli C, et al. Toll-like receptors2, -3and -4prime microglia but not astrocytes across central nervous system regions for ATP-dependent interleukin-1β release[J]. Sci Rep, 2014, 4：6824-6832.

东北林蛙发育早期TLR2的表达及免疫应答特征

The Expression and Immune Response Characteristics of TLR2 at the Early Development Stage of Rana chensinensis

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 11

编辑推荐

Metrics

本文评价

[1]	毛然然, 李小艳, 武瑶, 张丽珊, 林镇平, 林向民. 嗜水气单胞菌OprM蛋白的克隆表达与免疫保护作用评价[J]. 生物技术通报, 2019, 35(9): 244-248.
[2]	丁菲;徐宇虹;. 在体电脉冲基因导入技术研究进展[J]. , 2010, 0(10): 82-85.
[3]	周璨林;张富春;. 增强DNA疫苗免疫应答的新进展[J]. , 2006, 0(01): 22-25.
[4]	王颖. 基因免疫具有潜力[J]. , 1995, 0(01): 27-28.
[5]	陶冶;. Cellcor寻求FDA批准ALT[J]. , 1993, 0(10): 17-18.
[6]	孙雷心. 遗传免疫[J]. , 1992, 0(12): 12-13.
[7]	王颖;. Ribi Immunochem佐剂显着提高疟疾疫苗的免疫应答反应性[J]. , 1991, 0(10): 14-15.
[8]	王旭宁;. 1989年华尔街上筹资的成功者与失败者[J]. , 1990, 0(06): 37-38.
[9]	金人一;. 疟疾疫苗佐剂能改进rDNA疫苗[J]. , 1989, 0(01): 18-19.
[10]	王璋瑜;. Quidel R & D集中研究过敏抑制因子[J]. , 1987, 0(04): 9-9.
[11]	. 疫苗[J]. , 1986, 0(03): 88-89.