乙醇诱导对鼠李糖乳杆菌HCCB 20591代谢乙醛的影响

doi:10.13560/j.cnki.biotech.bull.1985.2017-0051

生物技术通报 ›› 2017, Vol. 33 ›› Issue (7): 133-137.doi: 10.13560/j.cnki.biotech.bull.1985.2017-0051

乙醇诱导对鼠李糖乳杆菌HCCB 20591代谢乙醛的影响

钟俭鸿¹,³,殷瑜²,³,陈代杰¹

1. 华东理工大学生物工程学院，上海 200237；
2. 上海交通大学药学院，上海 200240；
3. 上海来益生物药物研究开发中心有限责任公司，上海 201203

收稿日期:2017-01-24 出版日期:2017-07-11 发布日期:2017-07-11
作者简介:钟俭鸿，男，在读硕士研究生，研究方向：发酵工程微生物；E-mail：906269707@qq.com

Effect of Ethanol-induction on the Metabolism of Acetaldehyde in Lactobacillus rhamnosus HCCB 20591

ZHONG Jian-hong¹,³, YIN Yu²,³, CHEN Dai-jie¹

1. School of Biotechnology，East China University of Science and Technology，Shanghai 200237；
2. School of Pharmacy，Shanghai Jiao Tong University，Shanghai 200240；
3. Shanghai Laiyi Center for Biopharmaceutical R&D，Shanghai 201203

Received:2017-01-24 Published:2017-07-11 Online:2017-07-11

摘要/Abstract

摘要： 乙醛是酒类饮品中的风味物质，但乙醛过高不仅会影响酒类饮品的风味，还会对人体产生潜在的危害。从可食用的益生菌中筛选可以代谢乙醛的菌株，并探究活性菌株的乙醛代谢能力跟乙醇诱导的关系。通过静息细胞法筛选获得的鼠李糖乳杆菌HCCB 20591具有较强代谢乙醛的能力，且其代谢能力受乙醇诱导。当乙醇诱导浓度为3%，诱导时间为12 h时，乙醛转化率达到41.13%。GC-MS分析其代谢产物除了乙酸外，还生成了乙醇。采用Real time RT-PCR方法分析相关基因的转录水平，发现乙醛代谢是乙醇脱氢酶、乙醛脱氢酶和醛氧化还原酶综合催化作用的结果。

关键词: 鼠李糖乳杆菌, 乙醇诱导, 乙醛代谢, 综合催化作用

Abstract: Acetaldehyde is a flavor substance in alcoholic beverages. Excessive acetaldehyde not only affects the flavor of alcoholic beverages，but also causes health damages to human body. This study is to screen edible probiotic strains with the ability to metabolize acetaldehyde，and explore the relationship between acetaldehyde metabolism and ethanol-induction. Strain HCCB 20591 of Lactobacillus rhamnosus with strong acetaldehyde metabolism ability was obtained via a resting cell system，and its metabolic ability was induced by ethanol. When the ethanol concentration was 3% and the induction time was 12 h，the acetaldehyde conversion ratio by HCCB 20591 was 41.13%. GC-MS analysis of the acetaldehyde metabolites showed that in addition to acetic acid，ethanol was also generated by HCCB 20591. RT-PCR method was used to analyze the transcription level of the related genes. It was found that acetaldehyde metabolism was the combined catalytic result of alcohol dehydrogenase，aldehyde dehydrogenase and aldehyde oxidoreductase.

Key words: Lactobacillus rhamnosus, ethanol-induction, acetaldehyde metabolism, combined catalytic result

钟俭鸿,殷瑜,陈代杰. 乙醇诱导对鼠李糖乳杆菌HCCB 20591代谢乙醛的影响[J]. 生物技术通报, 2017, 33(7): 133-137.

ZHONG Jian-hong, YIN Yu, CHEN Dai-jie. Effect of Ethanol-induction on the Metabolism of Acetaldehyde in Lactobacillus rhamnosus HCCB 20591[J]. Biotechnology Bulletin, 2017, 33(7): 133-137.

参考文献

[1] Miyake T, Shibamoto T. Quantitative analysis of acetaldehyde in foods and beverages[J] . Journal of Agricultural and Food Chemistry, 1993, 41（11）：1968-1970.
[2] Sheridan MK, Elias RJ. Exogenous acetaldehyde as a tool for modulating wine color and astringency during fermentation[J] . Food Chem, 2015, 177：17-22.
[3] Liu SQ, Pilone GJ. An overview of formation and roles of acetaldehyde in wine making with emphasis on microbiological implications[J] . International Journal of Food Science & Technology, 2000, 35（1）：49-61.
[4] Paiano V, Bianchi G, Davoli E, et al. Risk assessment for the Italian population of acetaldehyde in alcoholic and non-alcoholic beverages[J] . Food Chem, 2014, 154：26-31.
[5] 朱梦旭, 范文来, 徐岩. 我国白酒蒸馏过程及原酒, 成品酒中乙醛的研究[J] . 食品与发酵工业, 2016（04）：6-11.
[6] Alvarez-Olmos MI, Oberhelman RA. Probiotic agents and infectious diseases：a modern perspective on a traditional therapy[J] . Clinical Infectious Diseases, 2001, 32（11）：1567-1576.
[7] Vitini E, Alvarez S, Medina M, et al. Gut mucosal immunostimulation by lactic acid bacteria[J] . Biocell, 2000, 24（3）：223-232.
[8] Tian F, Chi F, et al. Lactobacillus rhamnosus CCFM1107 treatment ameliorates alcohol-induced liver injury in a mouse model of chronic alcohol feeding[J] . J Microbiol, 2015, 53（12）：856-863.
[9] Nosova T, Jousimies-Somer H, Jokelainen K, et al. Acetaldehyde production and metabolism by human indigenous and probiotic Lactobacillus and Bifidobacterium strains[J] . Alcohol Alcohol, 2000, 35（6）：561-568.
[10] Kim JH, Kim HJ, Son JH, et al. Effect of Lactobacillus fermentum MG590 on alcohol metabolism and liver function in rats[J] . Journal of Microbiology and Biotechnology, 2003, 13（6）：919-925.
[11] Liu SQ, Asmundson RV, Holland R, et al. Acetaldehyde metabolism by Leuconostoc mesenteroides subsp. cremoris under stress conditions[J] . International Dairy Journal, 1997, 7（2）：175-183.
[12] 冯泳兰. 检验乙醛的一种简便方法[J] . 衡阳师范学院学报, 1990（3）：41-43.
[13] 贾青青, 邵威平, 辛秀兰, 等. 黑加仑果与果酒香气成分的GC-MS分析[J] . 中国酿造, 2014, 33（3）：141-146.
[14] Hayashi H, Sakamoto M, Benno Y. Phylogenetic analysis of the human gut microbiota using 16S rDNA clone libraries and strictly anaerobic culture-based methods[J] . Microbiol Immunol, 2002, 46（8）：535-548.
[15] Kelson TL, Mcvoy JRS, Rizzo WB. Human liver fatty aldehyde dehydrogenase：microsomal localization, purification, and biochemical characterization[J] . Biochim Biophys Acta, 1997, 1335（2）：99-110.
[16] 杨亚平. 乙醇脱氢酶基因和乙醛脱氢酶基因的克隆及表达[D] . 芜湖：安徽工程大学, 2013.
[17] 徐惠娟, Dürre P, 梁翠谊, 等. Clostridium ljungdahlii乙醛铁氧还蛋白氧化还原酶基因插入失活载体的构建[J] . 化工进展, 2012（S1）：523-523.
[18] Whitham JM, Tirado-Acevedo O, Chinn MS, et al. Metabolic response of Clostridium ljungdahlii to oxygen exposure[J] . Appl Environ Microbiol, 2015, 81（24）：8379.
[19] 朱敏, 李宝坤, 李开雄, 等. 乙醇胁迫对乳酸杆菌关键酶活力的影响[J] . 食品与发酵工业, 2015（1）：40-45.
[20] Ramos JL, Duque E, Rodríguezherva J, et al. Mechanisms for solvent tolerance in bacteria[J] . Journal of Biological Chemistry, 1997, 272（7）：3887-3890.

乙醇诱导对鼠李糖乳杆菌HCCB 20591代谢乙醛的影响

Effect of Ethanol-induction on the Metabolism of Acetaldehyde in Lactobacillus rhamnosus HCCB 20591

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 1

编辑推荐

Metrics

本文评价