香蕉花芽分化期抗枯萎病相关基因表达分析

doi:10.13560/j.cnki.biotech.bull.1985.2017-0071

摘要/Abstract

摘要： 通过对‘巴西蕉’和‘宝岛蕉’花芽分化期响应枯萎病菌4号生理小种（Fusarium oxysporum f. sp. cubense race 4，Foc4）侵染的转录组进行分析，获得了大量差异表达基因。本研究以‘巴西蕉’和‘宝岛蕉’花芽分化期根部组织为材料，采用实时荧光定量PCR（qPCR）方法测定了10个与特定抗病机制相关基因的表达变化。结果表明，10个基因在‘巴西蕉’和‘宝岛蕉’花芽分化过程中均有表达，但其表达量存在差异。与对照‘巴西蕉’相比，除laccase-4（A）基因在‘宝岛蕉’中的表达量明显下调外，laccase-4（B）、periplasmic beta-glucosidase precursor、glutathione-S-transferase Cla47、class III peroxidase 136 precursor、peroxidase N、similar to T-phylloplanin、snakin-1、putative chitinase及cellulose synthase catalytic subunit 12等9个基因的表达量均上调，其中peroxidase N基因的表达量呈极显著上调，相对表达量约为‘巴西蕉’的1 682倍。10个差异表达基因可能参与‘宝岛蕉’花芽分化期对病原菌的防卫反应，为进一步探讨‘宝岛蕉‘复杂的抗病防御反应分子机理奠定基础。

关键词: 香蕉枯萎病, 花芽分化期, 抗病相关基因, qPCR

Abstract: A large number of differentially expressed genes were obtained from transcriptome analysis of ‘Brazilian’ and ‘Formosana’ bananas infected by Fusarium oxysporum f. sp. cubense race 4（Foc4）at the flower bud differentiation stage. The expression variations of 10 disease-resistance related genes from diseased ‘Brazilian’ and ‘Formosana’ banana roots were detected by real-time quantitative PCR（qPCR）. The results showed that these 10 genes expressed in both ‘Brazilian’ and ‘Formosana’ at flower bud differentiation，but the expression levels of these genes varied. Compared with the control of ‘Brazilian’ banana，the expression levels of the nine genes（laccase-4（B），periplasmic beta-glucosidase precursor，glutathione-S-transferase Cla47，class III peroxidase 136 precursor，peroxidase N，similar to T-phylloplanin，snakin-1，putative chitinase，and ellulose synthase catalytic subunit 12）up-regulated except gene laccase-4（A）. Among them，the expression of peroxidase N significantly up-regulated，and the relative expression was 1 682 times as much as in ‘Brazilian’. These results suggested that 10 differentially expressed genes may be involved in defense responses against Foc4 at the flower bud differentiation stage of ‘Formosana’，which lay a foundation for the further study on molecular mechanisms of ‘Formosana’ defense against fusarium wilt.

Key words: banana fusarium wilt, flower bud differentiation stage, disease-resistance related gene, qPCR

王贵花,张欣,漆艳香,王宇光,彭军,谢艺贤. 香蕉花芽分化期抗枯萎病相关基因表达分析[J]. 生物技术通报, 2017, 33(7): 83-88.

WANG Gui-hua, ZHANG Xin ,QI Yan-xiang ,WANG Yu-guang ,PENG Jun ,XIE Yi-xian. Expression Analysis of Fusarium Wilt Resistance-Related Genes at the Flower Bud Differentiation Stage of Banana[J]. Biotechnology Bulletin, 2017, 33(7): 83-88.

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