一株分离自铀矿酸性浸出液霉菌的鉴定与碳源代谢分析

doi:10.13560/j.cnki.biotech.bull.1985.2017-0592

生物技术通报 ›› 2018, Vol. 34 ›› Issue (4): 208-213.doi: 10.13560/j.cnki.biotech.bull.1985.2017-0592

一株分离自铀矿酸性浸出液霉菌的鉴定与碳源代谢分析

刘欢¹, 徐玲玲^{1, 2}, 李江^{1, 2}

1. 东华理工大学,南昌 330013;
2. 东华理工大学省部共建核资源与环境国家重点实验室培育基地,南昌 330013

收稿日期:2017-07-17 出版日期:2018-04-20 发布日期:2018-05-04
作者简介:刘欢,女,硕士,研究生,研究方向：基因克隆;E-mail：804898610@qq.com
基金资助:
江西省教育厅（GJJ160563）,江西省科技厅科技计划项目（对外科技合作计划）（20161BBH80051）

Identification and Carbon Metabolic Analysis of a Mold Isolated from Uranium Acid Leaching Solution

LIU Huan¹, XU Ling-ling^{1, 2}, LI Jiang^{1, 2}

1. East China University of Technology,Nanchang 330013;
2. State Key Laboratory Brecding base of Nuclear Resoures and Environment,East China University of Tehnology,Nanchang 330013

Received:2017-07-17 Published:2018-04-20 Online:2018-05-04

摘要/Abstract

摘要： 旨在通过形态学观察、rDNA ITS序列分析以及Biolog微生物鉴定系统对霉菌进行鉴定,并通过计算平均吸光值（AWCD）了解霉菌的生长情况以及各类碳源的消耗量,运用数据分析霉菌的碳源利用情况。结果表明,结合rDNA ITS序列、形态学观察以及Biolog鉴定系统确定菌株为黑曲霉;比较8类碳源消耗情况,可知对氨基酸类、脂类、酸类的利用最多,根据碳源代谢情况列出15种最先利用碳源。鉴定菌株为黑曲霉,且该菌株的最适碳源为P-羟苯乙酸,可作为耐酸曲霉代谢研究的材料。

关键词: 形态学观察, rDNA ITS序列分析, 碳源代谢, Biolog微生物鉴定系统

Abstract: This study aims to identify a mold by morphological observation,rDNA ITS sequence analysis,and Biolog microbial identification system,to investigate the growth of the mold and its consumption of carbon source by calculating the average well color development（AWCD）,and to analyze carbon source utilization of the mold using data. Results revealed as such,combined rDNA ITS sequence with morphological observation and Biolog identification system,the strain was identified as Aspergillus niger. Compared the consumptions of 8 carbon sources,the types of amino acid,lipid and acid were the most widely used. According to the utilizations of carbon sources,15 first-used carbon sources were listed. The optimal carbon source of the strain was P-hydroxyphenylacetic acid,which could be used as a material for the research of acid-resistant Aspergillus metabolism.

Key words: morphological observation, rDNA ITS sequence analysis, carbon metabolic, Biolog microbial identification system

刘欢, 徐玲玲, 李江. 一株分离自铀矿酸性浸出液霉菌的鉴定与碳源代谢分析[J]. 生物技术通报, 2018, 34(4): 208-213.

LIU Huan, XU Ling-ling, LI Jiang. Identification and Carbon Metabolic Analysis of a Mold Isolated from Uranium Acid Leaching Solution[J]. Biotechnology Bulletin, 2018, 34(4): 208-213.

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一株分离自铀矿酸性浸出液霉菌的鉴定与碳源代谢分析

Identification and Carbon Metabolic Analysis of a Mold Isolated from Uranium Acid Leaching Solution

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