以抽油烟机废油为原料发酵产鼠李糖脂的研究

doi:10.13560/j.cnki.biotech.bull.1985.2017-0736

摘要/Abstract

摘要： 鼠李糖脂是一种具有巨大潜力的阴离子生物表面活性剂,可应用于石油、食品、农业、日化工业等领域。探讨以抽油烟机废油为碳源发酵产鼠李糖脂的可能性,以铜绿假单胞菌WB505为出发菌体,在7 L发酵罐中鼠李糖脂的产量达到12.3±0.52 g/L。利用基质辅助激光解析飞行时间质谱（MALDI-TOF MS）分析出所产鼠李糖脂的组成,结果显示其主要含Rha-C₁₀-C₁₀和Rha₂-C₁₀-C₁₀,其中单鼠李糖脂和双鼠李糖脂的总相对丰度分别为49.7%和50.3%。所产鼠李糖脂的临界胶束浓度（CMC）为45.0 mg/L,能将表面张力从60.5±0.81 mN/m降至25.3±0.68 mN/m,乳化系数E24均>60%,并且对苯的乳化系数达到80.3±0.85%。以抽烟机废油为底物生产鼠李糖脂降低底物成本,为抽油烟机废油提供一种循环再利用处理方式。

关键词: 生物表面活性剂, 鼠李糖脂, 铜绿假单胞菌, 抽油烟机废油, 临界胶束浓度

Abstract: Rhamnolipid（RL）is a kind of anionic biosurfactant with great potential and could be applied in the fields of petroleum,food,agriculture,and daily chemicals industry. This study explores the feasibility of producing rhamnolipid by fermentation with waste oil from range hood. Pseudomonas aeruginosa WB505 was used as the starting strain,and by which the yield of rhamnolipid in 7 L fermenter reached 12.3±0.52 g/L. Matrix-assisted laser desorption time-of-flight mass spectrometry was applied to analyze the composition of produced RL. Results showed that the main contents were Rha-C₁₀-C₁₀ and Rha₂-C₁₀-C₁₀,and the total abundance of mono-rhamnolipid and di-rhamnolipid were 49.7% and 50.3%,respectively. Critical micelle concentration（CMC）of the produced RL was 45.0 mg/L and it reduced the surface tension of water from 60.5±0.81 mN/m to 25.3±0.68 mN/m. E24 for six kinds of common hydrocarbons were higher than 60%,and E24 for benzene reached 80.3±0.85%. Therefore,using waste oil from range hood as substrate for RL production can reduce the capital cost of substrate,and provides a recycling treatment for waste oil from range hood.

Key words: biosurfactant, rhamnolipid, Pseudomonas aeruginosa, waste oil from range hood, critical micelle concentration

张婧波,吴剑荣,蒋芸,杨迪,詹晓北. 以抽油烟机废油为原料发酵产鼠李糖脂的研究[J]. 生物技术通报, 2018, 34(5): 195-200.

ZHANG Jing-bo, WU Jian-rong, JIANG Yun, Yang Di, ZHAN Xiao-bei. Fermentative Production of Rhamnolipid Using Waste Oil from Range Hood as Substrate[J]. Biotechnology Bulletin, 2018, 34(5): 195-200.

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