HFSC标记在阿尔巴斯绒山羊毛囊及毛囊干细胞中的表达

doi:10.13560/j.cnki.biotech.bull.1985.2017-0885

生物技术通报 ›› 2018, Vol. 34 ›› Issue (5): 201-205.doi: 10.13560/j.cnki.biotech.bull.1985.2017-0885

HFSC标记在阿尔巴斯绒山羊毛囊及毛囊干细胞中的表达

乃门塔娜¹,张燕军¹,刘东军²,李金泉¹

1. 内蒙古农业大学动物科学学院,呼和浩特 010018;
2. 内蒙古大学哺乳动物生殖生物学及生物技术教育部重点实验室,呼和浩特010070

收稿日期:2017-10-22 出版日期:2018-05-26 发布日期:2018-06-07
作者简介:乃门塔娜,女,博士,在站博后,研究方向：绒山羊绒毛生长机理;E-mail：naimentana@163.com
基金资助:
中国博士后科学基金资助项目（2017M613263XB）

Expression of HFSC Marker in the Arbas Cashmere Goat Hair Follicle and Hair Follicle Stem Cells

NIMANTANA¹, ZHANG Yan-jun¹, LIU Dong-jun², LI Jin-quan¹

1. College of Animal Science,Inner Mongolia Agricultural University,Huhehot 010018;
2. Key Laboratory of Mammalian Reproductive Biology and Biotechnology of Ministry of Education,Inner Mongolia University,Huhehot 010070

Received:2017-10-22 Published:2018-05-26 Online:2018-06-07

摘要/Abstract

摘要： 旨在检测最广泛应用的毛囊干细胞标记在阿尔巴斯绒山羊毛囊组织中的表达情况,为今后内蒙古绒山羊毛囊组织学和细胞学领域相关研究提供依据。以阿尔巴斯绒山羊为研究对象,对其进行Krt15、Krt19、CD34和Sox9等的冰冻切片和细胞免疫组化实验。以上4个标记均在外根鞘中表达;Krt15、Krt19在隆突部表达,在隆突部下端不连续表达;CD34在隆突部和隆突部下端都有较强的表达;Sox9主要集中在隆突部表达。Krt15、Krt19、CD34和Sox9共同表达的部位为毛囊隆突部,因此可结合表达部位特征选用为阿尔巴斯绒山羊毛囊隆突部来源的毛囊干细胞鉴定标记。

关键词: 阿尔巴斯绒山羊, 冰冻切片, 细胞免疫组化, 鉴定标记

Abstract: This study is aimed to detect the expression of widely-used hair follicle stem cell（HFSC）markers in the hair follicle of Arbas Cashmere goat,and to provide experimental evidence for further research on histology and cytology of hair follicle in Inner Mongolia Cashmere goat. Using Arbas Cashmere goat as study object,frozen section and immunohistochemistry of Krt15,Krt19,CD34 and Sox9 were performed. Results showed that the above-mentioned four markers all expressed in the outer root sheath;Krt15 and Krt19 positively expressed in the bulge region and non-contiguously in the suprabulbar region;CD34 had a strong expression in the bulge and suprabulbar region;Sox9 expression focused in the bulge region. Conclusively,Krt15,Krt19,CD34 and Sox9 all expressed in the bulge region of the hair follicle,thus they can be used as special markers of Arbas Cashmere goat hair follicle stem cells from bulge region according to their expressed location.

Key words: Arbas Cashmere goat, frozen section, cellular immunohistochemistry, identification marker

乃门塔娜,张燕军,刘东军,李金泉. HFSC标记在阿尔巴斯绒山羊毛囊及毛囊干细胞中的表达[J]. 生物技术通报, 2018, 34(5): 201-205.

NIMANTANA, ZHANG Yan-jun, LIU Dong-jun, LI Jin-quan. Expression of HFSC Marker in the Arbas Cashmere Goat Hair Follicle and Hair Follicle Stem Cells[J]. Biotechnology Bulletin, 2018, 34(5): 201-205.

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HFSC标记在阿尔巴斯绒山羊毛囊及毛囊干细胞中的表达

Expression of HFSC Marker in the Arbas Cashmere Goat Hair Follicle and Hair Follicle Stem Cells

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