生物技术通报 ›› 2017, Vol. 33 ›› Issue (3): 144-150.doi: 10.13560/j.cnki.biotech.bull.1985.2017.03.021

• 研究报告 • 上一篇    下一篇

利用杨木水解液制备细菌纤维素

陈慧慧, 刘玉, 王慧梅   

  1. 齐鲁工业大学 制浆造纸科学与技术教育部重点实验室,济南 250353
  • 收稿日期:2016-07-08 出版日期:2017-03-26 发布日期:2017-03-07
  • 作者简介:陈慧慧,女,硕士研究生,研究方向:植物资源化学工程与木质纤维基功能材料;E-mail:chhuihui2016054@163.com
  • 基金资助:
    国家自然科学基金项目(31270626)

Employing Poplar Wood Hydrolysate to Prepare Bacterial Cellulose

CHEN Hui-hui, LIU Yu, WANG Hui-mei   

  1. Key Laboratory of Pulp and Paper Science & Technology of Ministry of Education,Qilu University of Technology,Jinan 250353
  • Received:2016-07-08 Published:2017-03-26 Online:2017-03-07

摘要: 选用杨木木屑水解液为发酵基质,利用木醋杆菌发酵制备细菌纤维素。研究还原糖浓度、初始pH值、接种量、发酵温度和发酵时间对细菌纤维素产量、持水性和复水率的影响。结果表明,生产细菌纤维素的适宜条件为:杨木木屑水解液还原糖浓度为2.5%,初始pH为6.0,接种量为6%,发酵时间6 d,发酵温度30℃,在此条件下,细菌纤维素产量为3.14 g/L,持水性为98.72%,复水率为86.69%。采用扫描电镜、红外光谱、热失重分析法等技术分析了所制备的细菌纤维素的结构与性能,结果表明合成产物为超微细网状结构,且产物中含有的基团与纤维素结构相符合。

关键词: 杨木木屑水解液, 扫描电镜, 红外光谱, 热失重分析法

Abstract: Selecting the hydrolysate of poplar wood sawdust for fermentation substrate,we used Acetobacter xylinum to prepare bacterial cellulosee,and further studied the effect of reducing sugar concentration,the initial pH,inoculum size,fermentation temperature,and fermentation time on production,water holding capacity,and rehydration rate of bacterial cellulose. The results showed that the production of bacterial cellulose was 3.14 g/L,water holding capacity of bacterial cellulose was 98.72%,and bacterial cellulose rehydration was 86.69% when reducing sugar concentration of hydrolyzed poplar wood sawdust was 2.5%,the initial pH was 6.0,inoculum size was 6%,fermentation time was 6 d,and fermentation temperature was 30℃.The detection results of scanning electron microscope,infrared spectroscopy,and thermo-gravimetric analysis indicated that the structure of synthetic product was super fine mesh and the groups of the product corresponded to the structure of cellulose.

Key words: hydrolysate of poplar wood sawdust, scanning electron microscope, infrared spectroscopy, thermo-gravimetric analysis