保护酶PPO在木薯种质抗螨中的功能初步研究

doi:10.13560/j.cnki.biotech.bull.1985.2017.04.018

生物技术通报 ›› 2017, Vol. 33 ›› Issue (4): 143-148.doi: 10.13560/j.cnki.biotech.bull.1985.2017.04.018

保护酶PPO在木薯种质抗螨中的功能初步研究

梁晓¹, 卢芙萍¹, 卢辉¹, 伍春玲¹, 曹宪红², 陈青¹

1. 中国热带农业科学院环境与植物保护研究所农业部热带作物有害生物综合治理重点实验室海南省热带作物病虫害生物防治工程技术研究中心海南省热带农业有害生物监测与控制重点实验室,海口 571101；
2. 海南大学环境与植物保护学院,海口 570228

收稿日期:2016-08-21 出版日期:2017-04-25 发布日期:2017-04-25
作者简介:梁晓,男,博士,助理研究员,研究方向：生物化学与分子生物学及作物抗虫性;E-mail：liangxiaozju@126.com
基金资助:
国家木薯产业技术体系害虫防控岗位科学家专项（CAR S-12-hncq）（CARS-12-hncq）

Preliminary Study on the Function of Polyphenol Oxidase in Cassava Resistance to Mite

LIANG Xiao¹, LU Fu-ping¹, LU Hui¹, WU Chun-ling¹, CAO Xian-hong², CHEN Qing¹

1. Environment and Plant Protection Institute,China Academy of Tropical Agricultural Sciences/Laboratory of Pests Comprehensive Governance for Tropical Crops,Ministry of Agriculture/Hainan Key Laboratory for Monitoring and Control of Tropical Agricultural Pests/Hainan Engineering Research Center for Biological Control of Tropical Crops Diseases and Insect Pests,Haikou 571101;
2. College of Environment and Plant Protection,Hainan University,Haikou 570228

Received:2016-08-21 Published:2017-04-25 Online:2017-04-25

摘要/Abstract

摘要： 目前尚不了解保护酶PPO（polyphenol oxidase）在木薯种质抗螨中的作用,为证实PPO的抗螨功能,本研究通过定量PCR和酶学试验手段分析了朱砂叶螨取食抗、感螨木薯种质后,PPO的基因表达量和酶活分别在木薯和朱砂叶螨中的变化情况。结果表明,一方面,朱砂叶螨取食1 d和8 d后,感螨木薯种质BRA900体内MePPO的表达量和PPO总酶活仅分别是为害前的0.99、1.02 倍和1.05、1.03倍,而在抗螨木薯种质C1115体内则分别较为害前提高1.78、1.74倍和1.74、1.72倍,显著高于感螨木薯水平;另一方面,朱砂叶螨取食感螨种质BRA900 1 d和8 d后,保护酶TcPPO的基因表达量和PPO酶活分别是取食前的0.98、0.97倍和0.98、1.10倍,而取食抗螨木薯种质C1115 1 d和8 d后分别降低至为害前的0.52、0.64倍和0.54、0.57倍,显著低于取食感螨木薯水平。上述结果初步证实了保护酶PPO在木薯种质抗螨中的功能。

关键词: PPO, 朱砂叶螨, 木薯种质, 抗螨功能

Abstract: So far,the function of polyphenol oxidase（PPO）in cassava resistance to mite has not been understood clearly. In order to validate this function,we used quantitative real-time PCR（qPCR）and enzymatic assay to determine the changes of expression and activities of PPO in either cassava or Tetranychus cinnabarinus after the mite-resistant and mite-susceptible cassava cultivars were damaged by T. cinnabarinus. The results showed that,in one hand,compared to those in the same leaves before damaged,the transcriptions of MePPO and total activities of PPO only increased to 0.99-,1.02-fold and 1.05-,1.03-fold while the mite-susceptible cassava cultivar BRA900 was damaged by T. cinnabarinus for 1 d and 8 d,respectively. However,the corresponding values in 1 d- and 8 d-damaged leaves of mite-resistant cassava C1115 increased to 1.78-,1.74-fold and 1.74-,1.72-fold,respectively,thus significantly higher than those of mite-susceptible cassava. In the other hand,compared with those before feeding,the expressions of TcPPOand activities of PPO in T. cinnabarinus were 0.98-,0.97-fold and 0.98-,1.10-fold,respectively after feeding on BRA900 for 1 d and 8 d;while the corresponding values in T. cinnabarinus feeding on C1115 decreased to 0.52-,0.64-fold and 0.54-,0.57-fold,respectively. This study preliminarily validated the function of PPO in cassava resistance to T. cinnabarinus.

Key words: polyphenol oxidase, Tetranychus cinnabarinus, cassava, resistance to mite

梁晓, 卢芙萍, 卢辉, 伍春玲, 曹宪红, 陈青. 保护酶PPO在木薯种质抗螨中的功能初步研究[J]. 生物技术通报, 2017, 33(4): 143-148.

LIANG Xiao, LU Fu-ping, LU Hui, WU Chun-ling, CAO Xian-hong, CHEN Qing. Preliminary Study on the Function of Polyphenol Oxidase in Cassava Resistance to Mite[J]. Biotechnology Bulletin, 2017, 33(4): 143-148.

参考文献

[1] Cock JH. Cassava：a basic energy source in the tropics[J]. Science, 1982, 218（4574）：755-762.
[2] 伍薇, 柯佑鹏. 中国木薯产业发展现状及前景展望[J]. 中国热带农业, 2011, 1（3）：6-9.
[3] 陈青, 卢芙萍, 黄贵修, 等. 木薯害虫普查及其安全性评估[J]. 热带作物学报, 2010, 31（5）：819-827.
[4] Smith CM. Plant resistance to insects[M]. A fundamental approach：John Wiley and Sons Ltd, 1989.
[5] Taylor N, Chavarriaga M, Raemakers K, et al. Development and application of transgenic technologies in cassava[J]. Plant Molecular Biology, 2004, 56（4）：671-688.
[6] Felton GW, Donato KK, Broadway RM, et al. Impact of oxidized plant phenolics on the nutritional quality of dietary protein to a noctuid herbivore, Spodoptera exigua[J]. Journal of Insect Physiology, 1992, 38（4）：277-285.
[7] Yang ZW, Duan XN, Jin S, et al. Regurgitant derived from the tea geometrid Ectropis obliqua suppresses wound-induced polyphenol oxidases activity in tea plants[J]. Journal of Chemical Ecology, 2013, 39（6）：744-751.
[8] 王丹, 陈亮. 茶树对茶尺蠖抗性机制研究[J]. 茶叶科学, 2014, 34（6）：541-547.
[9] Han Y, Wang Y, Bi JL, et al. Constitutive and induced activities of defense-related enzymes in aphid-resistant and aphid-susceptible cultivars of wheat[J]. Journal of Chemical Ecology, 2009, 35（2）：176-182.
[10] 陈青, 张银东. 3 种氧化酶与辣椒抗蚜性的相关性[J] . 热带作物学报, 2004, 25（3）：42-46.
[11] 李迁, 卢芙萍, 陈青, 等. 木薯种质对朱砂叶螨的抗性评价[J]. 热带作物学报, 2015, 36（1）：143-151.
[12] Lu FP, Chen Q, Chen ZS, et al. Effects of heat stress on development, reproduction and activities of protective enzymes in Mononychellus mcgregori[J]. Experimental and Applied Acarology, 2014, 63（2）：267-284.
[13] Xu J, Duan X, Yang J, et al. Enhanced reactive oxygen species scavenging by overproduction of superoxide dismutase and catalase delays postharvest physiological deterioration of cassava storage roots[J]. Plant Physiology, 2013, 161（3）：1517-1528.
[14] Pfaffl MW. A new mathematical model for relative quantification in real-time RT-PCR[J]. Nucleic Acids Research, 2001, 29（9）：e45-e45.
[15] Ray PD. Huang BW, Tsuji Y. Reactive oxygen species（ROS）homeostasis and redox regulation in cellular signaling[J]. Cell Signal, 2012, 24（5）：981-990.
[16] Marco H, Gregg AH. Host plant-specific remodeling of midgut physiology in the generalist insect herbivore Trichoplusia ni[J]. Insect Biochemistry and Molecular Biology, 2014, 50（3）：58-67.
[17] Juan MA, Bernardus CJ. Schimmel, Joris JG, et al. Spider mites suppress tomato defenses downstream of jasmonate and salicylate independently of hormonal crosstalk[J]. New Phytologist, 2015, 205（2）：828-840.
[18] Gregg AH and Georg J. Plant immunity to insect herbivores[J]. Annual Review of Plant Biology, 2008, 59（1）：41-66.
[19] Franzen, LD, Gutsche AR, Heng-Moss T, et al. Physiological and biochemical responses of resistant and susceptible wheat to injury by Russian wheat aphid[J]. Journal of Economic Entomology, 2007, 100（5）：1692-1703.
[20] 张春妮. 甘蓝苗期对桃蚜抗性的生化机制研究[D]. 杨陵：西北农林大学, 2005.
[21] Krishnan N, Kodrík D. Antioxidant enzymes in Spodoptera littoralis（Boisduval）, are they enhanced to protect gut tissues during oxidative stress[J]. Journal of Insect Physiology, 2006, 52（1）：11-20.
[22] Barbehenn RV. Gut-based antioxidant enzymes in a polyphagous and a graminivorous grasshopper[J]. Journal of Chemical Ecology, 2002, 28（7）：1329-1347.

保护酶PPO在木薯种质抗螨中的功能初步研究

Preliminary Study on the Function of Polyphenol Oxidase in Cassava Resistance to Mite

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