氮肥对甘蔗叶片内生固氮菌nifH基因表达的影响

doi:10.13560/j.cnki.biotech.bull.1985.20170002

生物技术通报 ›› 2017, Vol. 33 ›› Issue (7): 100-106.doi: 10.13560/j.cnki.biotech.bull.1985.20170002

氮肥对甘蔗叶片内生固氮菌nifH基因表达的影响

李佳慧¹,袁丹¹,陆建明¹,杨丽涛¹,李杨瑞²,邢永秀¹

1. 广西大学农学院亚热带农业生物资源保护与利用国家重点实验室，南宁 530004；
2. 中国农业科学院甘蔗研究中心农业部广西甘蔗生物技术与遗传改良重点实验室广西农业科学院广西甘蔗遗传改良重点实验室，南宁 530007

收稿日期:2017-01-11 出版日期:2017-07-11 发布日期:2017-07-11
作者简介:李佳慧，女，硕士研究生，研究方向：代谢植物学；E-mail：1558779830@qq.com
基金资助:
国家自然科学基金项目（3156100093、31471449），广西八桂学者和特聘专家专项基金（2013），国家现代农业产业技术体系广西甘蔗创新团队项目（gjnytxgxcxtd-03），广西农业科学院团队项目（2015YT02）

Effects of Nitrogen Fertilizer on the Expression of nifH Gene of Endophytic Azotobacter in Sugarcane Leaves

LI Jia-hui¹, YUAN Dan¹, LU Jian-ming¹, YANG Li-tao¹, LI Yang-rui² ,XING Yong-xiu¹

1. State Key Laboratory of Conservation and Utilization of Subtropical Agro-Bioresources，Agricultural College，Guangxi University，Nanning 530004；
2. Sugarcane Research Center，Chinese Academy of Agricultural Sciences/Key Laboratory of Sugarcane Biotechnology and Genetic ImprovementGuangxi，Ministry of Agriculture/ Guangxi Academy of Agricultural Sciences/Guangxi Key Laboratory of Sugarcane Genetic Improvement，Nanning 530007

Received:2017-01-11 Published:2017-07-11 Online:2017-07-11

摘要/Abstract

摘要： 为了探究氮肥对甘蔗叶片内生固氮菌nifH基因表达的影响，从RNA和蛋白质水平，采用RT-PCR和Western blot 等技术分析施氮和不施氮处理下3个甘蔗品种叶片中nifH基因表达的差异性及对固氮酶相对表达量的影响。结果表明，在大田生长的甘蔗，在施氮肥和对照处理的叶片内，都可检测到固氮菌nifH基因在基因水平和蛋白质水平的表达。施氮处理会抑制或降低叶片内固氮菌的nifH基因的表达，但有利于nifH在蛋白质水平的表达，3个甘蔗品种内生固氮菌nifH基因的相对表达量都是施氮处理下较高，尤其是甘蔗品种ROC22，处理与对照间达到了显著差异。

关键词: 甘蔗, 内生固氮菌, 固氮酶, nifH基因

Abstract: In order to investigate the effects of nitrogen fertilizer on the expression of nifH gene of endophytic diazotrophs in sugarcane leaves，we carried out a field experiment with and without nitrogen fertilization，and used RT-PCR and Western blot technology to analyze the differences of nifH expression and the relative expression quantity of nitrogenase in 3 sugarcane varieties，GXB9，ROC22 and GT11. The results showed that the expressions of nifH gene in endogenous azotobacter were detected at gene and protein levels in the leaves of sugarcane growing in field both with nitrogen fertilization and without nitrogen fertilization. Nitrogen fertilization treatment inhibited or decreased the expression of nifH gene in nitrogen-fixing bacteria，but it was beneficial to the expression of nifH at the protein level. The relative expressions of the nifH gene in the leaves of three sugarcane cultivars were all higher under nitrogen application than the control without nitrogen application；especially，the difference was significant for cultivar ROC22.

Key words: sugarcane, endogenous azotobacter, nitrogenase, nifH

李佳慧,袁丹,陆建明,杨丽涛,李杨瑞,邢永秀. 氮肥对甘蔗叶片内生固氮菌nifH基因表达的影响[J]. 生物技术通报, 2017, 33(7): 100-106.

LI Jia-hui, YUAN Dan, LU Jian-ming, YANG Li-tao, LI Yang-rui ,XING Yong-xiu. Effects of Nitrogen Fertilizer on the Expression of nifH Gene of Endophytic Azotobacter in Sugarcane Leaves[J]. Biotechnology Bulletin, 2017, 33(7): 100-106.

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氮肥对甘蔗叶片内生固氮菌nifH基因表达的影响

Effects of Nitrogen Fertilizer on the Expression of nifH Gene of Endophytic Azotobacter in Sugarcane Leaves

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