SNaPshot技术检测滩羊双羔群体中与多胎性状相关SNP研究

doi:10.13560/j.cnki.biotech.bull.1985.2019-0167

摘要/Abstract

摘要： 对我国地方品种滩羊双羔群体进行SNP检测和多胎性状相关性研究,促进SNPs在双羔家系分子育种中的应用。从实验前期SNP筛选的基础上,利用候选基因飞行质谱法最终确定3种基因13个SNP位点作为候选位点,对滩羊双羔群体的多胎位点进行多态性检测,分析群体差异性,筛选多羔候选基因。关联分析表明：13个位点中有5个位点与多胎性能有关,3种遗传模型也被证实,但BMPR1B基因构建的单倍型却与多胎性能无关。本研究旨在为滩羊双羔分子标记本品种选种选配等分子辅助育种技术提供参考,保护与可持续利用地方家养动物稀有种质遗传资源,加快优质多胎肉羊的发展。

关键词: 滩羊, 双羔, SNaPshot, 多胎

Abstract: The SNPs（Single Nucleotide Polymorphism）detection and correlation study of multiparous traits were carried out on the double-lamb group of Tan sheep,aiming at promoting the application of SNPs in the genetic breeding of double-lamb in China. On the basis of SNPs screening at the previous studies,13 SNPs sites of 3 genes were determined as candidate sites by the flight mass spectrometry of candidate genes. Population differences were analyzed and multiparous candidate genes were screened by detecting polymorphism of the double-lamb group of Tan sheep. The association analyses showed that 5 of 13 loci were associated with multiparous performance,3 genetic models were confirmed,whereas haplotype constructed by BMPR1B gene was not associated with multiparous performance. The study is aimed at providing reference for molecule-assisted breeding techniques,such as molecule-marker species selection and breed selection for double-lamp group of Tan sheep in the protection and also a sustainable utilization of rare germplasm genetic resources of indigenous domestic animal,therefore accelerating the breeding progress of high quality multiparous mutton sheep.

Key words: Tan sheep, double-lamb, SNaPshot, prolificacy

田得红, 刘思嘉, 丁宁, 李雪, 赵凯. SNaPshot技术检测滩羊双羔群体中与多胎性状相关SNP研究[J]. 生物技术通报, 2019, 35(10): 152-161.

TIAN De-hong, LIU Si-jia, DING Ning, LI Xue, ZHAO Kai. Research on SNP Associated with Multiparous Trait in Double-lamb of Tan Sheep by SNaPshot Assay[J]. Biotechnology Bulletin, 2019, 35(10): 152-161.

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