FEN1酶介导的功能核酸生物传感器的研究进展

doi:10.13560/j.cnki.biotech.bull.1985.2019-0224

摘要/Abstract

摘要： 瓣状核酸内切酶-1（Flap endonuclease 1,FEN1）是一种可以识别三碱基重叠结构（三核酸）并对其进行切割,释放出5'-flap片段的结构特异性酶,并且有着高效稳定的切割效率。基于此种特性,通过不同的信号输出方式,FEN1酶现被用于DNA、RNA、病毒等放大检测中。首先对FEN1酶的发现、性质以及作用方面做了相应介绍,然后根据所检测的靶物质不同,对FEN1酶所介导的生物传感器进行分类,主要包括对单核苷酸多态性的检测、甲基化检测、基因型检测、RNA检测、病毒检测、肿瘤检测和微生物检测等。此外,对FEN1酶与纳米材料的结合以及体内表征及治疗也进行了较为详细的介绍。同时,还对传感器之间的原理、灵敏度、特异性及适用领域等方面进行比较和优缺点的简单评价。最后,对FEN1酶所介导的生物传感器的中存在的不足,以及未来的发展方向进行了展望,旨在为今后研发更便携、更灵敏、更准确的FEN1功能核酸生物传感器提供理论参考。

关键词: FEN1酶, 三核酸, 信号放大, 生物传感器, 核酸内切酶

Abstract: Flap endonuclease 1（FEN1）is a structure-specific enzyme that can recognize and efficiently and stably cleave the triplex DNA structures to release 5'-flap fragments. Due to its specific characteristic,FEN1 is nowadays widely used for amplification detection of DNA,RNA,and viruses through different signal output modes. This review firstly provides an overview regarding the discovery history,specific properties and comprehensive effects of FEN1,followed by detailed classification of the FEN1-mediated biosensors in terms of the target substances including the detections of single nucleotide polymorphism,methylation,genotype,RNA,virus,tumor and microbe. In addition,this review describes the uses of FEN-1 for in vivo characterization and treatment by combining with nanomaterials. Moreover,the review compares and evaluates different FEN1-mediated biosensors with respect to working principle,sensitivity,specificity and application fields. Finally,the review highlights the challenges and the future research perspectives of FEN1-mediated biosensors,aiming at facilitating the development of a more convenient,sensitive and accurate FEN1-mediated biosensor for functional nucleic acid detection in the future.

Key words: FEN1, triplex DNA structure, signal amplification, biosensor, endonuclease

孙雨阁, 李宸葳, 杜再慧, 许文涛. FEN1酶介导的功能核酸生物传感器的研究进展[J]. 生物技术通报, 2020, 36(4): 208-224.

SUN Yu-ge, LI Chen-wei, DU Zai-hui, XU Wen-tao. Research Progress on FEN1-mediated Functional Nucleic Acid Biosensors[J]. Biotechnology Bulletin, 2020, 36(4): 208-224.

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