核酸适配体侧流层析分析技术在真菌毒素检测中的应用

doi:10.13560/j.cnki.biotech.bull.1985.2019-0830

摘要/Abstract

摘要： 侧流层析分析技术（Lateral flow chromatography analysis technique,LFAs）具有简单、快速、经济、高效等特点,可以非实验室条件下快速呈现检测结果。核酸适配体作为新型“化学抗体”,具有体外合成,易于修饰和批次差异小等特点。适配体侧流层析分析技术是将侧流层析技术和适配体结合而研发的一种新型快速检测技术。可用于食品安全、环境和临床分析等领域的污染物的定性、半定量和定量检测。综述了近年来利用适配体结合荧光、纳米金和磁珠等标记物,通过光学、比色、表面增强拉曼散射的检测方式在真菌毒素快速检测领域的应用,旨在为适配体侧流层析生物传感技术的设计提供参考。

关键词: 侧流层析分析技术, 适配体, 生物传感器, 快速检测, 真菌毒素

Abstract: Lateral flow chromatography analysis technique（LFAs）characterized as simple,rapid,economic,and efficient one and can quickly presents the test results under the laboratory condition. New chemical antibody,nucleic acid aptamer body becomes a novel identifying component because of its in vitro screening,easy synthesis and modification,small batch difference and so on. Aptamer lateral flow chromatography analysis technique is the novel and fast detection technique of combing lateral flow tomography technology with aptamer. The LFAs is mainly used for qualitative,semi-quantitative and quantitative detection of contaminants in food safety,environment and clinical analysis. This paper reviews the LFA susing the aptamer combined with markers such as fluorescent,nano-gold and magnetic beads,as well as their applications in the rapid detection of fungal toxins through optics,colorimetry,surface-enhanced raman scattering,which aims at providing a reference in designing aptamer lateral flow chromatography analysis biological sensing technology.

Key words: lateral flow chromatography analysis technique, aptamer, biosensor, rapid detection, fungal toxin

赵颖, 王楠, 陆安祥, 冯晓元, 郭晓军, 栾云霞. 核酸适配体侧流层析分析技术在真菌毒素检测中的应用[J]. 生物技术通报, 2020, 36(8): 217-227.

ZHAO Ying, WANG Nan, LU An-xiang, FENG Xiao-yuan, GUO Xiao-jun, LUAN Yun-xia. Application in the Detection of Fungal Toxins by Nucleic Acid Aptamer Lateral Flow Chromatography Analysis Technique[J]. Biotechnology Bulletin, 2020, 36(8): 217-227.

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