生物技术通报 ›› 2020, Vol. 36 ›› Issue (5): 145-149.doi: 10.13560/j.cnki.biotech.bull.1985.2019-0827

• 研究报告 • 上一篇    下一篇

苏姜猪OLR1基因多态性及其与肉质性状的关联分析

任善茂, 王温慧, 陶勇   

  1. 江苏农牧科技职业学院,泰州 225300
  • 收稿日期:2019-09-06 出版日期:2020-05-26 发布日期:2020-06-03
  • 作者简介:任善茂,女,博士,副教授,研究方向:动物营养与饲料科学;E-mail:825587062@qq.com
  • 基金资助:
    江苏高校“青蓝工程”项目(2017(15)),江苏农牧科技职业学院科研项目(NSF201602)

Polymorphism of OLR1 Gene and Its Association with Meat Quality Traits in Sujiang Pigs

REN Shan-mao, WANG Wen-hui, TAO Yong   

  1. Jiangsu Agri-animal Husbandry Vocational College,Taizhou 225300
  • Received:2019-09-06 Published:2020-05-26 Online:2020-06-03

摘要: 探讨OLR1基因在苏姜猪群内的遗传多态性,以及该基因多态对苏姜猪猪肉质性状的影响。采用PCR-RFLP技术检测OLR1基因在苏姜猪试验群体中的PstⅠ酶切遗传多态性,运用单因素方差分析方法分析了该多态位点对苏姜猪肉质性状的影响。结果发现,苏姜猪试验群体OLR1基因内含子5区域内发现一个PstⅠ酶切多态性,检测到CC、CD和DD三种基因型,多态信息含量呈现中度多态性。CC型与DD型个体的肌肉失水率、大理石纹间的差异达到显著水平(P<0.05),CD型个体用色差仪测得的b值显著高于DD型(P<0.05)。因此,检测到的OLR1基因PCR-RFLP-PstⅠ多态性与大理石纹等肉质性状存在着显著的相关关系,可以作为肉质性状候选基因在苏姜猪的持续选育中加以应用。

关键词: 苏姜猪, OLR1基因, PCR-RFLP-PstⅠ, 肉质性状, 关联分析

Abstract: This work is to investigate the polymorphism of lipoprotein-receptor-l(OLR1)gene and its association with the meat quality traits in Sujiang pigs. The Pst genetic polymorphism of OLR1 gene in Sujiang pigs was detected by PCR-RFLP,and the association between OLR1 genotype and meat quality traits was analyzed with one-way ANOVA. The results showed that Pst Ⅰ polymorphism in the intron 5 of OLR1 gene in the experimental population of Sujiang pig was found,and three genotypes(CC,CD and DD)were detected. The polymorphism information content was moderate. The difference of the water loss rate and marbling between CC and DD genotypes of OLR1 gene were significant(P<0.05),and the b value of meat color of CD genotype by color difference meter was significantly higher than that of DD genotype(P<0.05). Therefore,the detected PCR-RFLP-Pst I polymorphism of OLR1 gene is significantly correlated with some meat traits such as marbling,and it could be used as a potential candidate gene of meat quality traits in the continuous breeding of Sujiang pigs.

Key words: Sujiang pig, OLR1 gene, PCR-RFLP-Pst Ⅰ, meat quality trait, association analysis