好氧反硝化菌Achromobacter sp.L16的脱氮特性

doi:10.13560/j.cnki.biotech.bull.1985.2019-0978

摘要/Abstract

摘要： 在垃圾渗滤液中分离出一株异养硝化-好氧反硝化菌L16,经过形态观察和16S rDNA基因序列分析鉴定为Achromobacter sp.,L16对氨氮和硝酸盐氮的去除率分别为61.94%和98.40%。对菌株好氧反硝化和异养硝化培养条件进行优化结果表明：L16在以硝酸盐为氮源、柠檬酸钠为碳源、C/N为20、培养温度为30℃、培养转速为150 r/min条件下硝酸盐氮去除率为99.74%,总氮去除率58.90%。L16在以氨氮为氮源、柠檬酸钠为碳源、C/N为20、培养温度为30℃、培养转速为200 r/min条件下氨氮去除率提高到93.41%,总氮去除率86.33%。优化后L16具有高效的异养硝化-好氧反硝化能力,可将氮素大部分转化为气体和菌体胞内氮,具有潜在的实际废水应用价值。

关键词: 好氧反硝化, 异养硝化, 无色杆菌

Abstract: A heterotrophic nitrification-aerobic denitrification strain L16 was isolated from landfill leachate and identified as Achromobacter sp. by morphological observation and 16s rDNA gene sequence analysis. The rate of removing ammonia nitrogen was 61.94% and that of removing nitrate nitrogen was 98.40%. The culture conditions of aerobic denitrification and heterotrophic denitrification of strain L16 was optimized by single factor experiment. The optimized results of aerobic denitrification and heterotrophic nitrification of strain L16 showed that L16 was cultured with nitrate as nitrogen source,sodium citrate as carbon source,C/N 20,culture temperature 30℃,rotate speed 150 r/min,and the degradation rate of nitrate nitrogen and the rate of removing total nitrogen was 99.74% and 58.90%, respectively. Under the conditions of ammonia nitrogen as nitrogen source,sodium citrate as carbon source,C /N 20,culture temperature 30℃ and rotate speed 200 r/min,and the degradation rate of ammonia nitrogen increased to 93.41% and the removal rate of total nitrogen was 86.33%. The optimized strain L16 has high efficiency of heterotrophic nitrification-aerobic denitrification,converts the most of the nitrogen into gas and intracellular nitrogen,and thus has potential value of practical application in wastewater treatment.

Key words: erobic denitrification, heterotrophic nitrification, Achromobacter sp.

李思琦, 杨静丹, 刘琳, 刘二佳, 王晓慧. 好氧反硝化菌Achromobacter sp.L16的脱氮特性[J]. 生物技术通报, 2020, 36(6): 93-101.

LI Si-qi, YANG Jing-dan, LIU Lin, LIU Er-jia, WANG Xiao-hui. Denitrification Characteristics of Aerobic Denitrifying Bacteria Achromobacter sp. L16[J]. Biotechnology Bulletin, 2020, 36(6): 93-101.

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