杜氏盐藻促生菌株的分离与鉴定

doi:10.13560/j.cnki.biotech.bull.1985.2019-1264

生物技术通报 ›› 2020, Vol. 36 ›› Issue (5): 169-175.doi: 10.13560/j.cnki.biotech.bull.1985.2019-1264

杜氏盐藻促生菌株的分离与鉴定

段露露, 杭伟, 程宇娇, 崔红利, 王计平, 李润植

山西农业大学分子农业与生物能源研究所,太谷 030801

收稿日期:2019-12-25 出版日期:2020-05-26 发布日期:2020-06-03
作者简介:段露露,女,硕士研究生,研究方向：植物生理生态;E-mail：964619274@qq.com
基金资助:
国家自然科学基金项目（31201266,31401430）,农业农村部“948”项目（2014-Z39）,山西省煤基重大科技专项（FT-2014-01）,山西省留学基金项目（2015-064）,山西省重点研发重点项目（201603D312005）,山西省重点研发一般项目（201803D31063）

Isolation and Identification of the Growth-promoting Bacteria from Pbycosphere of Dunaliella salina

DUAN Lu-lu, HANG Wei, CHENG Yu-jiao, CUI Hong-li, WANG Ji-ping, LI Run-zhi

Institute of Molecular Agriculture and Bioenergy,Shanxi Agricultural University,Taigu 030801

Received:2019-12-25 Published:2020-05-26 Online:2020-06-03

摘要/Abstract

摘要： 拟通过探究藻际微生物对微藻生长及代谢产物积累的影响,筛选出促进微藻生长的促生菌株。以杜氏盐藻（Dunaliella salina）Ds-SXYC-2为试材,分离鉴定盐藻藻际环境中的共生菌株,进一步构建藻菌（1∶1）共培养体系、测试盐藻生长及代谢产物积累等表型。结果显示,从杜氏盐藻藻际环境分离获得5株共生菌株,经16S rDNA分子鉴定,属于3个菌属。菌株B1与B2为涅斯捷连科氏菌（Nesterenkonia）,菌株B3与B4为盐单胞菌（Halomonas）,菌株B5为海杆菌（Marinobacter）。5株共生菌株对杜氏盐藻的生长均有促进作用,菌株B3能显著促进杜氏盐藻生长及代谢产物的积累。共培养15 d后,杜氏盐藻生物量达到2.3 g/L,比对照组增加了28.9%,叶绿素a的含量达到4.61 mg/L,比对照组增加了36.3%,β-胡萝卜素比对照组提高了56.4%。盐藻多糖、蛋白质、总脂含量分别比对照组增加了34.8%、71.2%和37.6%。菌株B3盐单胞菌可以作为促进杜氏盐藻生长及代谢产物累积的优势菌株,进一步构建共培养体系可应用于杜氏盐藻的商业生产。

关键词: 杜氏盐藻, 藻际细菌, 藻菌共培养体系

Abstract: Through exploring the influence of phycosphere microorganisms on the microalgae growth and metabolite accumulation,the predominant growth-promoting strains were screened. The symbiotic bacterial strains from the phycosphere of Dunaliella salina Ds-SXYC-2 were isolated and identified,and 1∶1 algal-bacteria co-culture system was constructed,and the growth of D. salina and the accumulation of its metabolite were measured. The results showed that 5 symbiotic bacterial strains were isolated from the phycosphere of D. salina,and they belonged to 3 genera,including Nesterenkonia（Strain B1 and B2）,Halomonas（strain B3 and B4）,and Marinobacter（Strain B5）. All the 5 strains promoted the growth of D. salina. Among them,strain B3 significantly promoted the growth and the accumulation of metabolites. After 15 d of co-culture,the biomass of D. salina reached 2.3 g/L,which was higher than that of the control without bacteria and increased by 28.9%. The content of chlorophyll a（4.61 mg/L）and beta-carotene increased by 36.3% and 56.4%,respectively,in contrast to the control. The contents of intracellular polysaccharide,proteins and total lipids increased by 34.8%,71.2% and 37.6%,respectively,compared with the control group. Halomonas strain B3 could be used as the dominant strain to promote the growth and metabolite accumulation of D. salina,and the co-culture system could be constructed and applied to the commercial production of D. salina.

Key words: Dunaliella salina, bacterial flora in phycosphere, algal-bacteria co-culture system

段露露, 杭伟, 程宇娇, 崔红利, 王计平, 李润植. 杜氏盐藻促生菌株的分离与鉴定[J]. 生物技术通报, 2020, 36(5): 169-175.

DUAN Lu-lu, HANG Wei, CHENG Yu-jiao, CUI Hong-li, WANG Ji-ping, LI Run-zhi. Isolation and Identification of the Growth-promoting Bacteria from Pbycosphere of Dunaliella salina[J]. Biotechnology Bulletin, 2020, 36(5): 169-175.

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杜氏盐藻促生菌株的分离与鉴定

Isolation and Identification of the Growth-promoting Bacteria from Pbycosphere of Dunaliella salina

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