作物根际微生物组重组构建技术体系探讨

doi:10.13560/j.cnki.biotech.bull.1985.2020-0796

摘要/Abstract

摘要： 作物根际微生物组在作物的生长发育和抵抗逆境胁迫方面扮演着非常重要的角色。根际微生物组的改良、重组及构建对于提高作物抗逆性能具有重要意义。以提高作物抗病能力为例,初步探讨了三种作物根际微生物组重组构建技术体系,分别为土壤菌悬液接种法、作物间作/套种法及土壤记忆法,从理论层面和实际应用方面阐述了技术体系的特点和应用可能,为未来农业可持续发展中根际微生物组的应用提供依据。

关键词: 根际微生物组, 作物抗病能力, 土壤菌悬液接种, 作物间作/套种, 土壤记忆

Abstract: The rhizosphere microbiome is of vital importance in growth and stress-resistance of crops. The improvement,reorganization and construction of rhizosphere microbiome are essential for improving the stress resistance of crops. In this study,three technical systems of reorganization and construction of rhizosphere microbiome are discussed preliminarily,including soil suspension inoculation,intercropping of crops and the application of soil memory. The characteristics and application feasibilities of the technical systems are explained from the theoretical level and practical application,thereby providing evidences for the application of rhizosphere microbiome in further sustainable development of agriculture.

Key words: rhizosphere microbiome, disease-resistance of crops, soil suspension inoculation, intercropping, soil memory

孙雨, 常晶晶, 田春杰. 作物根际微生物组重组构建技术体系探讨[J]. 生物技术通报, 2020, 36(9): 25-30.

SUN Yu, CHANG Jing-jing, TIAN Chun-jie. Technical Systems of Reorganization and Construction of Crop Rhizosphere Microbiome[J]. Biotechnology Bulletin, 2020, 36(9): 25-30.

参考文献

[1] Berendsen RL, Pieterse CM, Bakker PA.The rhizosphere microbiome and plant health[J]. Trends Plant Sci, 2012, 17:478-486.
[2] Muller DB, Vogel C, Bai Y, et al.The plant microbiota:systems-level insights and perspectives[J]. Annual Review of Genetics, 2016, 50:211-234.
[3] Khosla R.Science breakthroughs 2030:transforming food and agriculture research[J]. CSA News, 2018, 63:14-15.
[4] 刘双江. 微生物组:新机遇, 新天地[J]. 微生物学报, 2017, 57(6):791.
Liu SJ.Microbiome:New opportunities, new worlds[J]. Acta Microbiologica Sinica, 2017, 57(6):791.
[5] Sasse J, Martinoia E, Northen T.Feed your friends:do plant exudates shape the root microbiome?[J]. Trends Plant Sci, 2018, 23:25-41.
[6] Mbodj D, Effa-Effa B, Kane A, et al.Arbuscular mycorrhizal symbiosis in rice:establishment, environmental control and impact on plant growth and resistance to abiotic stresses[J]. Rhizosphere, 2018, 8:12-26.
[7] Zhang R, Vivanco JM, Shen Q.The unseen rhizosphere root-soil-microbe interactions for crop production[J]. Current Opinion in Microbiology, 2017, 37:8-14.
[8] Berendsen RL, Vismans G, Yu K, et al.Disease-induced assemblage of a plant-beneficial bacterial consortium[J]. ISME J, 2018, 12:1496-1507.
[9] Szoboszlay M, Lambers J, Chappell J, et al.Comparison of root system architecture and rhizosphere microbial communities of Balsas teosinte and domesticated corn cultivars[J]. Soil Biology and Biochemistry, 2015, 80:34-44.
[10] Shenton M, Iwamoto C, Kurata N, et al.Effect of wild and cultivated rice genotypes on rhizosphere bacterial community composition[J]. Rice(N Y), 2016, 9:42.
[11] Chen S, Waghmode TR, Sun R, et al.Root-associated microbiomes of wheat under the combined effect of plant development and nitrogen fertilization[J]. Microbiome, 2019, 7:136.
[12] Mendes LW, Raaijmakers JM, de Hollander M, et al. Influence of resistance breeding in common bean on rhizosphere microbiome composition and function[J]. ISME Journal, 2018, 12:212-224.
[13] Leff JW, Lynch RC, Kane NC, et al.Plant domestication and the assembly of bacterial and fungal communities associated with strains of the common sunflower, Helianthus annuus[J]. New Phytol, 2017, 214:412-423.
[14] 乔雪娟. 微生物在植物病虫害防治上的应用[J]. 林业科技情报, 2019, 51(4):17-18.
Qiao XJ.Application of microorganisms in the control of plant diseases and insect pests[J]. Forestry Science and Technology Information, 2019, 51(4):17-18.
[15] 邢磊, 赵圣国, 郑楠, 等. 未培养微生物分离培养技术研究进展[J]. 微生物学通报, 2017, 44(12):3053-3066.
Xing L. Zhao SG, Zhen N, et al.Advance in isolation and culture techniques of uncultured microbes:a review[J]. Microbiology, 2017, 44(12):3053-3066.
[16] Liu Y, Qin Y, Bai Y.Reductionist synthetic community approaches in root microbiome research[J]. Current Opinion in Microbiology. 2019, 49:97-102.
[17] Yan Y, Kuramae EE, Klinkhamer PG, et al.Revisiting the dilution procedure used to manipulate microbial biodiversity in terrestrial systems[J]. Applied and Environmental Microbiology. 2015, 81:4246-4252.
[18] Panke-Buisse K, Poole AC, Goodrich JK, et al.Selection on soil microbiomes reveals reproducible impacts on plant function[J]. ISME Journal. 2015, 9:980-989.
[19] Chang C, Chen W, Luo S, et al.Rhizosphere microbiota assemblage associated with wild and cultivated soybeans grown in three types of soil suspensions[J]. Archives of Agronomy and Soil Science. 2018, 65:74-87.
[20] 李集勤, 陈俊标, 袁清华, 等. 客土改良对植烟土壤营养及烟草青枯病的影响[J]. 中国烟草科学, 2017, 38(1):48-52.
Li JQ, Chen JB, Yuan QH, et al.Effects of alien earth soil-improving on soil nutrient status and tobaccobacterial wilt[J].
21 Chinese Tobacco Science, 2017, 38(1):48-52.
[21] van de Voorde TFJ, van der Putten WH, Bezemer TM. Soil inoculation method determines the strength of plant-soil interactions[J]. Soil Biology and Biochemistry, 2012, 55:1-6.
[22] Hassani MA, Duran P, Hacquard S.Microbial interactions within the plant holobiont[J]. Microbiome, 2018, 6:58.
[23] Barto EK, Weidenhamer JD, Cipollini D, et al.Fungal superhighways:do common mycorrhizal networks enhance below ground communication?[J]. Trends Plant Sci, 2012, 17:633-637.
[24] Jin X, Shi Y, Wu F, et al.Intercropping of wheat changed cucumber rhizosphere bacterial community composition and inhibited cucumber Fusarium wilt disease[J]. Scientia Agricola, 2020, 77.
[25] Perez-Jaramillo JE, Mendes R, Raaijmakers JM.Impact of plant domestication on rhizosphere microbiome assembly and functions[J]. Plant Mol Biol, 2016, 90:635-644.
[26] Mendes LW, Mendes R, Raaijmakers JM, et al.Breeding for soil-borne pathogen resistance impacts active rhizosphere microbiome of common bean[J]. ISME Journal, 2018, 12:3038-3042.
[27] Tian L, Shi S, Nasir F, et al.Comparative analysis of the root transcriptomes of cultivated and wild rice varieties in response to Magnaporthe oryzae infection revealed both common and species-specific pathogen responses[J]. Rice(N Y), 2018, 11:26.
[28] Brooker RW, Bennett AE, Cong WF, et al.Improving intercropping:a synthesis of research in agronomy, plant physiology and ecology[J]. New Phytol, 2015, 206:107-117.
[29] Wei Z, Gu Y, Friman VP, et al. Initial soil microbiome composition and functioning predetermine future plant health[J]. Science Advances, 2019, 5:eaaw0759 .
[30] Raaijmakers JM, Mazzola M.Soil immune responses[J]. Science, 2016, 352:1392-1393.
[31] Yuan J, Zhao J, Wen T, et al.Root exudates drive the soil-borne legacy of aboveground pathogen infection[J]. Microbiome, 2018, 6:156.
[32] 黄新琦, 蔡祖聪. 土壤微生物与作物土传病害控制[J]. 中国科学院院刊, 2017, 32:593-600.
Huang XQ, Cao ZC.Soil microbes and control of soil-borne diseases[J]. Bulletin of the Chinese Academy of Sciences, 2017, 32:593-600.
[33] Lapsansky ER, Milroy AM, Andales MJ, et al.Soil memory as a potential mechanism for encouraging sustainable plant health and productivity[J]. Current Opinion in Biotechnology, 2016, 38:137-142.
[34] Bettenfeld P, Fontaine F, Trouvelot S, et al.Woody plant declines. What’s wrong with the microbiome?[J]. Trends Plant Sci, 2020, 25:381-394.
[35] Liu J, Sui Y, Yu Z, et al.High throughput sequencing analysis of biogeographical distribution of bacterial communities in the black soils of northeast China[J]. Soil Biology and Biochemistry, 2014, 70:113-122.
[36] Meisner A, De Deyn GB, de Boer W, et al. Soil biotic legacy effects of extreme weather events influence plant invasiveness[J]. Proceedings of the National Academy of Sciences of the United States of America, 2013, 110:9835-9838.
[37] Zhang T, Chen HYH, Ruan H.Global negative effects of nitrogen deposition on soil microbes[J]. ISME Journal, 2018, 12:1817-1825.
[38] 徐莹莹, 王俊河, 刘玉涛, 等. 耕作与秸秆还田方式对连作玉米田根际微生物及酶活性的影响[J]. 黑龙江农业科学, 2018, 7:1-3.
Xu YY, Wang JH, Liu YT, et al.Effects of tillage and straw returning on microorganism and enzyme activity in continuous cropping corn field[J]. Heilongjiang Agricultural Sciences, 2018, 7:1-3.
[39] Fu L, Penton CR, Ruan Y, et al.Inducing the rhizosphere microbiome by biofertilizer application to suppress banana Fusarium wilt disease[J]. Soil Biology and Biochemistry, 2017, 104:39-48.
[40] Hamel C, Gan Y, Sokolski S, et al.High frequency cropping of pulses modifies soil nitrogen level and the rhizosphere bacterial microbiome in 4-year rotation systems of the semiarid prairie[J]. Applied Soil Ecology, 2018, 126:47-56.