不同抗、感水稻品种对褐飞虱肠道菌群的影响

doi:10.13560/j.cnki.biotech.bull.1985.2020-0797

摘要/Abstract

摘要：

本研究旨在探究取食不同抗性水稻品种对褐飞虱肠道菌群多样性及丰度的影响。将生长一致的3龄褐飞虱若虫分别置于高抗水稻品种RHT、高感水稻品种TN1以及中等抗性水稻品种ZH11,取食1 d和3 d后,分别对褐飞虱肠道进行取样,提取总DNA并通过HiSeq 2500平台对肠道细菌16S rRNA-V4区进行测序。对测序结果进行分析,统计不同样本中肠道微生物操作分类单元（operational taxonomic unit,OTU）数量,并在不同等级的分类阶元上统计细菌组成、丰度及Alpha多样性。对取食3种不同抗性水稻品种的褐飞虱肠道菌群特有及共有OTUs进行统计,并对丰度较高及显著差异菌群进行统计分析。褐飞虱每个肠道样本获得70 000余条有效序列,根据序列相似性聚类成一定数目的OTUs。褐飞虱分别取食RHT、TN1、ZH11水稻 1 d后的样本中,共有的OTUs数目为213个,特有的OTUs数目分别为424、77和130个。取食3 d后的样本中,共有的OTUs数目为217个,特有的OTUs数目分别为140、162和63个。对不同菌群含量进行分析发现,不动杆菌属Acinetobacter及沃尔巴克氏体属Wolbachia是含量较高的两类优势菌,其中不动杆菌属在取食RHT的褐飞虱肠道中的含量低于取食TN1及ZH11的褐飞虱肠道中的含量。同时,对319个属的细菌物种在分别取食RHT,TN1和ZH11的褐飞虱样本中的含量进行分析,发现了8类差异显著的物种。褐飞虱能够通过改变肠道细菌组成响应不同抗性水稻品种。研究结果为进一步挖掘褐飞虱体内肠道菌群在响应水稻抗性过程以及开发基于微生物资源进行褐飞虱防控提供参考。

关键词: 褐飞虱, 抗虫品种, 感虫品种, 水稻, 16S rRNA, 肠道菌群

Abstract:

This study aims to investigate the effects of resistant and sensitive rice varieties on the diversity and abundance of the gut microbiota of brown planthopper（BPH）. The 3^rd instar BPH nymphs were feeding on resistant rice variety RHT,susceptible rice variety TN1,and medium-resistant rice variety ZH11,and the intestines of BPH were sampled after 1 and 3 d. Total DNA were extracted and sequenced through the HiSeq 2500 platform for 16S rRNA-V4 region of bacteria in the gut. The sequencing results were analyzed to count the number of OTUs（Operational taxonomic unit）in different samples. The species composition,abundance,and the alpha diversity were counted at different levels of taxonomies. The specific and common bacteria of BPH after feeding with three different rice varieties were counted. The bacteria with high abundance and significant difference were analyzed. More than 70 000 valid sequences were obtained from each sample,and a certain number of OTUs were clustered based on sequence similarity. There were 213 common OTUs and 424,77,and 130 unique OTUs in 1 d samples of BPH after feeding on RHT,TN1,and ZH11,respectively,and 217 common OTUs and 140,162,63 unique OTUs in 3 d samples. The Acinetobacter and Wolbachia were two dominant gut microbiota species,and the content of Acinetobacter in the brown planthopper that fed on RHT was lower than that fed on TN1 and ZH11. Among 319 genera of bacteria in BPH,8 types of bacteria demonstrated significant differences in BPH after feeding on RHT,TN1 and ZH11 rice,respectively,which may be closely related to the BPH feeding on different resistant rice varieties. BPH can respond to different resistant rice varieties by changing the intestinal bacterial composition. This study lays a foundation for further exploring the intestinal microbiota of the BPH and their response to different rice varieties,and also provides a reference for controlling brown planthoppers using microbial resources.

Key words: Nilaparvata lugens, resistant variety, susceptible variety, rice, 16S rRNA, gut microbiota

李海超, 谢飞, 张园琦, 关若冰. 不同抗、感水稻品种对褐飞虱肠道菌群的影响[J]. 生物技术通报, 2021, 37(3): 1-9.

LI Hai-chao, XIE Fei, ZHANG Yuan-qi, GUAN Ruo-bing. Effects of Resistant and Sensitive Rice Varieties on Gut Microbiota of Nilaparvata lugens[J]. Biotechnology Bulletin, 2021, 37(3): 1-9.

图/表 4

参考文献 36

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Sample	observed species	chao	ace	shannon	simpson	coverage
mean（RHT.1D）	388.66667	395.44461	395.11502	1.45736	0.51068	0.99972
SD（RHT.1D）	192.10501	190.41556	191.33268	0.23077	0.18074	0.00009
mean（TN1.1D）	192.33333	233.66591	267.93055	1.92246	0.31738	0.99946
SD（TN1.1D）	93.32917	65.20748	27.16002	0.99904	0.17186	0.00036
mean（ZH11.1D）	251.66667	255.6381	254.37961	2.03145	0.35986	0.99988
SD（ZH11.1D）	50.73789	51.35924	50.2521	0.50533	0.11153	0.00002
mean（RHT.3D）	253	260.98931	259.054	1.46526	0.50256	0.99977
SD（RHT.3D）	76.5441	76.10176	79.18294	0.34932	0.08544	0.00014
mean（TN1.3D）	282.33333	296.16628	292.6945	1.62864	0.37817	0.99963
SD（TN1.3D）	39.10669	43.00768	41.60751	0.04288	0.07577	0.00015
mean（ZH11.3D）	181	216.55371	220.79586	0.91988	0.57233	0.99945
SD（ZH11.3D）	93.95212	61.23732	58.88241	0.57029	0.28043	0.00014

Sample	observed species	chao	ace	shannon	simpson	coverage
mean（RHT.1D）	388.66667	395.44461	395.11502	1.45736	0.51068	0.99972
SD（RHT.1D）	192.10501	190.41556	191.33268	0.23077	0.18074	0.00009
mean（TN1.1D）	192.33333	233.66591	267.93055	1.92246	0.31738	0.99946
SD（TN1.1D）	93.32917	65.20748	27.16002	0.99904	0.17186	0.00036
mean（ZH11.1D）	251.66667	255.6381	254.37961	2.03145	0.35986	0.99988
SD（ZH11.1D）	50.73789	51.35924	50.2521	0.50533	0.11153	0.00002
mean（RHT.3D）	253	260.98931	259.054	1.46526	0.50256	0.99977
SD（RHT.3D）	76.5441	76.10176	79.18294	0.34932	0.08544	0.00014
mean（TN1.3D）	282.33333	296.16628	292.6945	1.62864	0.37817	0.99963
SD（TN1.3D）	39.10669	43.00768	41.60751	0.04288	0.07577	0.00015
mean（ZH11.3D）	181	216.55371	220.79586	0.91988	0.57233	0.99945
SD（ZH11.3D）	93.95212	61.23732	58.88241	0.57029	0.28043	0.00014