铜绿假单胞菌适配体的获得及应用

doi:10.13560/j.cnki.biotech.bull.1985.2023-0336

摘要/Abstract

摘要：

铜绿假单胞菌是一种专性需氧的革兰氏阴性菌，是引起人类机会性感染的最主要的微生物，对人类造成多种健康威胁。因此，快速、灵敏、特异的检测对预防及治疗铜绿假单胞菌感染十分关键。目前常规的检测方法仍存在耗时久、成本高、操作难度大等问题，因此需要更加高效经济的检测方法。适配体是一种从随机文库中筛选出来的寡核苷酸，能够形成多种复杂的二级结构，近年来，由于其易于合成和修饰，并且具有高亲和力和强特异性而受到了广泛的关注，也涌现出了大量基于适配体的铜绿假单胞菌检测和防治的研究。本文总结了目前铜绿假单胞菌适配体的筛选和裁剪方面的研究进展，并从铜绿假单胞菌的检测和防治两个方面对其适配体的应用进行了介绍，以期为铜绿假单胞菌适配体的开发和应用提供参考。

关键词: 铜绿假单胞菌, 适配体, 筛选, 裁剪, 检测, 抑菌, 治疗

Abstract:

Pseudomonas aeruginosa is an obligate aerobic Gram-negative bacterium, which is the most important microorganism causing opportunistic infections in humans and poses a variety of health threats to humans. Thus, rapid, sensitive and specific detection is crucial for the prevention and treatment of P. aeruginosa infection. At present, there are problems for conventional detection methods，such as time-consuming, high cost and difficult operation, thus more efficient and economical detection methods are needed. Aptamers are oligonucleotides screened from random libraries that can form a variety of complex secondary structures. In recent years, aptamers have attracted widespread attention due to their ease of synthesis and modification, high affinity and strong specificity, and numerous studies of detection, the prevention and treatment of P. aeruginosa based on aptamers have emerged. In this review, the current progresses in screening and tailoring of P. aeruginosa aptamers were summarized, and the application of its aptamers were introduced from two aspects of detection and control of P. aeruginosa, aiming to provide a reference for the development and application of P. aeruginosa aptamer.

Key words: Pseudomonas aeruginosa, aptamer, screening, tailoring, detection, antibacteria, treatment

刘星雨, 李洁, 朱龙佼, 李相阳, 许文涛. 铜绿假单胞菌适配体的获得及应用[J]. 生物技术通报, 2024, 40(1): 186-193.

LIU Xing-yu, LI Jie, ZHU Long-jiao, LI Xiang-yang, XU Wen-tao. Aptamer of Pseudomonas aeruginosa: Acquiring and Application[J]. Biotechnology Bulletin, 2024, 40(1): 186-193.

图/表 4

参考文献 37

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靶标 Target	筛选方式SELEX type	序列 Sequence（5'-3'）	K_d/（nmol·L^-1）	亲和力检测方式 Affinity detection method	长度Length/nt	参考文献Reference
全菌	Cell-SELEX	CCCCCGTTGCTTTCGCTTTTCCTTTCGCTTGT-GTTCGTTTCGTCCCTGCTTCCTTCCTTCCTTC-CTTTCTTG	17.27 ± 5.00	流式细胞术	72	[14]
全菌	Cell-SELEX	ATGCACTCTTCTATCGGTAGTTGAGGGTGCGG	12.5±2.4	流式细胞术	32	[15]
外毒素A	Decoy-SELEX	TGTACCGTCTGAGCGATTCGTACCATAGGGTGCTTTTCAAGGCCACACGTTAGTGTAAGCCAGTCAGTGTTAAGGAGTGC	4 200-4 500	表面等离子体共振	80	[16]
脂多糖	Capture-SELEX	CCTTCTAACAGAATGTTGTTAGATAGC	46.2±9.5	等热滴定量热	27	[17]
全菌	Cell-SELEX	GGGAGGAC^FGAU^FGC^FGGGAAC^FU^FGAAGAGU^FU^FU^FGAU^FC^FAU^FGGC^FU^FC^FAGAU^FU^FGAAC^FGC^FU^FGGC^FGGC^FAGAC^FGAC^FUC^FGC^FGC^FGA	—	—	73	[18]
全菌	WB-SELEX	TGGACCTTGCGATTGACAGCAGACATGAGTCAGGACGTGACCGCTGGACCTTGCGATTGACAGCAGACATGAGTCAGGAC	15.16±3.62	流式细胞术	80	[19]
全菌	Cell-SELEX	CCCCCGTTGCTTTCGCTTTTCCTTTCGCTTTTGTTCGTTTCGTCCCTGCTTCCTTTCTTG	14.55	地高辛标记适配体	60	[20]
丁酰基-高丝氨酸	Structure-switching SELEX	CCATCCACACTCCGCAAGTGGGGAGGGGAGAGACGACGATCCTGTGGGTTTTCTGCAGTGAGTCGTGTTTTCGACTTATTGCGTCGGCTGCCTCTACAT	28.47	流式细胞术	99	[21]

靶标 Target	筛选方式SELEX type	序列 Sequence（5'-3'）	K_d/（nmol·L^-1）	亲和力检测方式 Affinity detection method	长度Length/nt	参考文献Reference
全菌	Cell-SELEX	CCCCCGTTGCTTTCGCTTTTCCTTTCGCTTGT-GTTCGTTTCGTCCCTGCTTCCTTCCTTCCTTC-CTTTCTTG	17.27 ± 5.00	流式细胞术	72	[14]
全菌	Cell-SELEX	ATGCACTCTTCTATCGGTAGTTGAGGGTGCGG	12.5±2.4	流式细胞术	32	[15]
外毒素A	Decoy-SELEX	TGTACCGTCTGAGCGATTCGTACCATAGGGTGCTTTTCAAGGCCACACGTTAGTGTAAGCCAGTCAGTGTTAAGGAGTGC	4 200-4 500	表面等离子体共振	80	[16]
脂多糖	Capture-SELEX	CCTTCTAACAGAATGTTGTTAGATAGC	46.2±9.5	等热滴定量热	27	[17]
全菌	Cell-SELEX	GGGAGGAC^FGAU^FGC^FGGGAAC^FU^FGAAGAGU^FU^FU^FGAU^FC^FAU^FGGC^FU^FC^FAGAU^FU^FGAAC^FGC^FU^FGGC^FGGC^FAGAC^FGAC^FUC^FGC^FGC^FGA	—	—	73	[18]
全菌	WB-SELEX	TGGACCTTGCGATTGACAGCAGACATGAGTCAGGACGTGACCGCTGGACCTTGCGATTGACAGCAGACATGAGTCAGGAC	15.16±3.62	流式细胞术	80	[19]
全菌	Cell-SELEX	CCCCCGTTGCTTTCGCTTTTCCTTTCGCTTTTGTTCGTTTCGTCCCTGCTTCCTTTCTTG	14.55	地高辛标记适配体	60	[20]
丁酰基-高丝氨酸	Structure-switching SELEX	CCATCCACACTCCGCAAGTGGGGAGGGGAGAGACGACGATCCTGTGGGTTTTCTGCAGTGAGTCGTGTTTTCGACTTATTGCGTCGGCTGCCTCTACAT	28.47	流式细胞术	99	[21]