甜瓜CmRGLG基因家族鉴定及表达特性分析

doi:10.13560/j.cnki.biotech.bull.1985.2025-0307

生物技术通报 ›› 2025, Vol. 41 ›› Issue (12): 156-167.doi: 10.13560/j.cnki.biotech.bull.1985.2025-0307

甜瓜CmRGLG基因家族鉴定及表达特性分析

王亚萍¹(), 金兰¹, 郝金凤², 长明¹, 王艳丹¹, 高峰¹()

^1.内蒙古师范大学生命科学与技术学院内蒙古自治区高等学校蒙古高原生物多样性保护与可持续利用重点实验室，呼和浩特 010022
^2.内蒙古大学生命科学学院牧草与特色作物生物学教育部重点实验室，呼和浩特 010021

收稿日期:2025-03-21 出版日期:2025-12-26 发布日期:2026-01-06
通讯作者: 高峰，男，博士，副教授，研究方向：植物分子生物学；E-mail: imgaofeng@163.com
作者简介:王亚萍，女，硕士研究生，研究方向：生物化学与分子生物学；E-mail: imypwang@163.com
基金资助:
国家自然科学基金项目(31660577);国家自然科学基金项目(32360765);内蒙古师范大学引进高层次人才科研启动金专项资金资助(2025YJRC083);内蒙古师范大学学生科技创新项目(2025XSKC16)

Identification and Expression Characteristics Analysis of CmRGLG Gene Family in Melon

WANG Ya-ping¹(), JIN Lan¹, HAO Jin-feng², CHANG Ming¹, WANG Yan-dan¹, GAO Feng¹()

^1.College of Life Sciences and Technology, Inner Mongolia Normal University, Key Laboratory of Biodiversity Conservation and Sustainable Utilization for Mongolian Plateau in Higher Education Institutions of Inner Mongolia Autonomous Region, Hohhot 010022
^2.School of Life Sciences, Inner Mongolia University, Key Laboratory of Biology of Forage and Characteristic Crops, Ministry of Education, Hohhot 010021

Received:2025-03-21 Published:2025-12-26 Online:2026-01-06

摘要/Abstract

摘要：

目的 RGLG蛋白属于RING（Really Interesting New Gene）型E3泛素连接酶，通过泛素化降解其他蛋白参与植物生长发育和非生物胁迫响应。鉴定甜瓜CmRGLG基因家族（CmRGLGs）成员，并分析相关基因的表达模式，为进一步探究其潜在功能奠定理论基础。方法采用生物信息学方法对CmRGLGs染色体定位、基因结构及其编码蛋白的理化特性、系统进化、共线性关系、蛋白互作等方面进行分析，通过RT-qPCR对各成员在不同器官、不同激素浓度梯度和非生物胁迫处理后甜瓜幼叶中的表达水平进行解析。结果在甜瓜全基因组中共鉴定出6个CmRGLGs成员，根据各基因在染色体上的位置及排列顺序依次命名为CmRGLG1-CmRGLG6；所编码的蛋白均为亲水性蛋白，氨基酸数为364-596 aa；在进行系统进化分析时，CmRGLGs基因分属于不同的3个分支。种内与种间共线性分析表明，CmRGLGs中不存在基因重复事件，CmRGLG1、CmRGLG2、CmRGLG4、CmRGLG5在拟南芥、黄瓜、番茄中均存在共线基因；启动子区中存在与植物激素和非生物胁迫响应有关的顺式作用元件；蛋白互作网络预测分析提示，CmRGLGs相互作用的蛋白主要富集在泛素‒蛋白转移酶活性、蛋白质代谢、生物合成和有机环状化合物结合等途径；表达特性分析结果显示，CmRGLG5和CmRGLG6在茎中的表达量最低，CmRGLG1、CmRGLG2、CmRGLG3、CmRGLG4在花中的表达量较高，且各基因成员在不同植物激素和非生物胁迫中具有不同程度的表达。结论甜瓜CmRGLGs在不同植物激素处理条件下，多数成员的表达水平有下调趋势；在非生物胁迫处理条件下，多数成员的表达水平呈先上调再下调的趋势。

关键词: 甜瓜, 泛素连接酶, RGLG基因家族, 生物信息学, 蛋白互作, 植物激素, 非生物胁迫, 表达分析

Abstract:

Objective RGLG proteins belong to the RING (Really Interesting New Gene, RING) type E3 ubiquitin ligases, which participate in plant growth, development, and abiotic stress responses by ubiquitinating and degrading other proteins. By identifying the members of the CmRGLGs gene family (CmRGLGs) in melon and analyzing their expression patterns, this study lays a theoretical foundation for further investigation into their potential functions. Method Bioinformatics methods were employed to analyze the chromosomal localization, gene structure, physicochemical properties of encoded proteins, phylogenetic evolution, syntenic relationships, and protein-protein interactions of CmRGLGs. The expression levels of these genes in different organs, under varying phytohormone concentrations, and after abiotic stress treatments were analyzed using RT-qPCR in young melon leaves. Result A total of six CmRGLGs members (CmRGLG1-CmRGLG6) were identified in the melon genome, named based on their chromosomal positions. The encoded proteins were hydrophilic, with amino acid lengths ranging from 364 to 596 aa. Phylogenetic analysis classified CmRGLGs into three distinct branches. Intraspecific and interspecific collinearity analysis revealed no gene duplication events among CmRGLGs. CmRGLG1, CmRGLG2, CmRGLG4, and CmRGLG5 showed collinear genes in Arabidopsis thaliana, Cucumis sativus, and Solanum lycopersicum. The promoter regions contained cis-acting elements associated with phytohormones and abiotic stress responses. Protein-protein interaction network prediction revealed that CmRGLGs-interacting proteins were primarily enriched in ubiquitin-protein transferase activity, protein metabolism, biosynthesis, and organic cyclic compound binding pathways. Expression analysis showed that CmRGLG5 and CmRGLG6 were expressed in the stems at the lowest level, while CmRGLG1, CmRGLG2, CmRGLG3, and CmRGLG4 were more abundant in the flowers. Additionally, each gene presented varying expression under different phytohormone treatments and abiotic stress conditions. Conclusion Under different phytohormone treatments, most CmRGLG members in melon showed downregulated expression trends. Under abiotic stress treatments, the majority of members demonstrated an initial upregulation followed by downregulation in their expressions.

Key words: Cucumis melo L., ubiquitin ligase, RGLG gene family, bioinformatics, protein-protein interaction, phytohormones, abiotic stress, expression analysis

王亚萍, 金兰, 郝金凤, 长明, 王艳丹, 高峰. 甜瓜CmRGLG基因家族鉴定及表达特性分析[J]. 生物技术通报, 2025, 41(12): 156-167.

WANG Ya-ping, JIN Lan, HAO Jin-feng, CHANG Ming, WANG Yan-dan, GAO Feng. Identification and Expression Characteristics Analysis of CmRGLG Gene Family in Melon[J]. Biotechnology Bulletin, 2025, 41(12): 156-167.

图/表 11

表1 RT-qPCR引物序列

Table 1 RT-qPCR primer sequences

引物名称 Primer name	正向引物序列 Forward primer sequence （5′‒3′）	反向引物序列 Reverse primer sequence （5′‒3′）	产物大小 Product size （bp）
CmGAPDH	ATCATTCCTAGCAGCACTGG	TTGGCATCAAATATGCTTGACCTG	278
CmRGLG1	AGTGGGGTCATTATGGTTATCCG	CCTCTTTCTTGTCTCAGGGGCT	116
CmRGLG2	GGTCAATCCCCCCCTTAT	GCTCCTGTTACCTCCTCCAA	221
CmRGLG3	CTTGTTTCGGTTTTGGTGAT	CCAGGTGGTGTTTTAGGATTTC	262
CmRGLG4	ATATCTCCCCAGAGAGTTGCA	AAGACCAGGTAGGCTTTGAAT	97
CmRGLG5	CAAACTCCACAACAACCACGG	GAGATTAGATGACTCAAGTCCAGCG	123
CmRGLG6	CGATTCTCCTAATCCCTACCA	TTGCCCACCACTCTTCTCTA	280

图1 甜瓜CmRGLGs基因结构和保守基序分析A：基因结构；B：保守基序；C：基序序列徽标（每个字母的高度表示该位置氨基酸出现的频率）

Fig. 1 Gene structure and conservative motif analysis of CmRGLGs in melonA: Gene structure. B: Conserved motifs. C: Motif sequence logo (The height of each letter indicates the frequency of amino acid occurrence at that position)

图2 甜瓜与其他物种RGLG蛋白家族系统进化树Cm：甜瓜；At：拟南芥；Os：水稻；Pa：银白杨；Ta：小麦；Gs：野大豆；Gh：陆地棉；Bn：甘蓝型油菜；Hs：木槿；Zo：姜

Fig. 2 Phylogenetic tree of RGLG protein family in melon and other speciesCm: Cucumis melo; At: Arabidopsis thaliana; Os: Oryza sativa; Pa: Populus alba; Ta: Triticum aestivum; Gs: Glycine soja; Gh: Gossypium hirsutum; Bn: Brassica napus; Hs: Hibiscus syriacus; Zo: Zingiber officinale

图3 甜瓜CmRGLGs染色体定位

Fig. 3 Chromosomal localization of CmRGLGs in melon

图4 甜瓜CmRGLGs种内（A）与不同物种间（B）共线性分析灰色线条代表甜瓜基因组与参与分析物种之间的共线性区块；红色线条突出了RGLG基因对

Fig. 4 Intraspecific (A) and interspecific synteny (B) analysis of CmRGLGs in melonGray lines indicate collinear blocks between the melon genome and the genomes of the analyzed species; red lines highlight RGLG gene pairs

图5 甜瓜CmRGLGs启动子区顺式作用元件分析

Fig. 5 Cis-acting elements analysis in the promoter region of CmRGLGs in melon

图6 Cytoscape 3.10.2分析蛋白互作中KEGG和GO注释统计结果A：KEGG通路富集分析；B：GO功能富集分析（横坐标表示每个Term中富集基因所占百分比，柱状图末端数字表示对应Term的具体基因数量；纵坐标表示显著富集的GO Terms）。*P<0.05，**P<0.01

Fig. 6 Statistical results of KEGG and GO annotation in protein-protein interaction analysis by Cytoscape 3.10.2A: KEGG pathway enrichment analysis. B: GO functional enrichment analysis (X-axis: percentage of enriched genes per Term; numbers at the end of bars indicate the specific gene count in each Term; Y-axis: significantly enriched GO Terms). *P<0.05, **P<0.01

图7 甜瓜CmRGLGs蛋白相互作用网络六边形表示生物过程；三角形表示分子功能；圆形表示蛋白

Fig. 7 Protein-protein interaction network of CmRGLGs in melonHexagons indicate biological processes； triangles indicate molecular functions； circles indicate proteins

图8 甜瓜CmRGLGs在不同器官中的表达特性分析不同小写字母表示差异显著（P<0.05）。下同

Fig. 8 Analysis of expression characteristics of CmRGLGs in different organs of melonDifferent lowercase letters indicate significant differences (P<0.05). The same below

图9 甜瓜CmRGLGs在不同植物激素（A）和非生物胁迫处理（B）下的表达特性分析

Fig. 9 Analysis of expression characteristics of CmRGLGs under phytohormone (A) and abiotic stress treatments (B) in melon

图10 甜瓜果实转录组数据中CmRGLGs的表达特性分析35、40、45 DAP分别表示授粉后35、40、45 d

Fig. 10 Analysis of expression characteristics of CmRGLGs in the transcriptome data of melon fruits35, 40, and 45 DAP indicate 35, 40, and 45 d after pollination (DAP), respectively

参考文献 40

[1]	Lobaina DP, Tarazi R, Castorino T, et al. The ubiquitin-proteasome system (UPS) and viral infection in plants [J]. Plants, 2022, 11(19): 2476.
[2]	Chen X, Dou QP, Liu JB, et al. Targeting ubiquitin-proteasome system with copper complexes for cancer therapy [J]. Front Mol Biosci, 2021, 8: 649151.
[3]	Yang Q, Zhao JY, Chen D, et al. E3 ubiquitin ligases: styles, structures and functions [J]. Mol Biomed, 2021, 2(1): 23.
[4]	Lee JH, Kim WT. Regulation of abiotic stress signal transduction by E3 ubiquitin ligases in Arabidopsis [J]. Mol Cells, 2011, 31(3): 201-208.
[5]	Du Z, Zhou X, Li L, et al. plantsUPS: a database of plants’ ubiquitin proteasome system [J]. BMC Genomics, 2009, 10: 227.
[6]	Deshaies RJ, Joazeiro CAP. RING domain E3 ubiquitin ligases [J]. Annu Rev Biochem, 2009, 78: 399-434.
[7]	Saxena H, Negi H, Sharma B. Role of F-box E3-ubiquitin ligases in plant development and stress responses [J]. Plant Cell Rep, 2023, 42(7): 1133-1146.
[8]	Sharma B, Saxena H, Negi H. Genome-wide analysis of HECT E3 ubiquitin ligase gene family in Solanum lycopersicum [J]. Sci Rep, 2021, 11(1): 15891.
[9]	苌兴超, 王雪松, 张沿政, 等. 大豆GmPUB32基因的克隆及耐盐功能分析 [J]. 中国油料作物学报, 2021, 43(4): 638-647.
	Chang XC, Wang XS, Zhang YZ, et al. Cloning and salt resistance analysis of soybean GmPUB32 gene [J]. Chin J Oil Crop Sci, 2021, 43(4): 638-647.
[10]	徐焕焕, 张芳毓, 刘茜, 等. 番茄SlSL1重组蛋白的表达、纯化及体外泛素化活性检测 [J]. 合肥工业大学学报: 自然科学版, 2022, 45(8): 1130-1134.
	Xu HH, Zhang FY, Liu Q, et al. Expression, purification and in vitro ubiquitination analysis of tomato SlSL1 recombinant protein [J]. J Hefei Univ Technol Nat Sci, 2022, 45(8): 1130-1134.
[11]	Freemont PS. Ubiquitination: RING for destruction [J]. Curr Biol, 2000, 10(2): R84-R87.
[12]	Pan IC, Tsai HH, Cheng YT, et al. Post-transcriptional coordination of the Arabidopsis iron deficiency response is partially dependent on the E3 ligases RING domain ligase1 (RGLG1) and RING domain ligase2 (RGLG2) [J]. Mol Cell Proteom, 2015, 14(10): 2733-2752.
[13]	Stone SL, Hauksdóttir H, Troy A, et al. Functional analysis of the RING-type ubiquitin ligase family of Arabidopsis [J]. Plant Physiol, 2005, 137(1): 13-30.
[14]	Katoh S, Hong C, Tsunoda Y, et al. High precision NMR structure and function of the RING-H2 finger domain of EL5, a rice protein whose expression is increased upon exposure to pathogen-derived oligosaccharides [J]. J Biol Chem, 2003, 278(17): 15341-15348.
[15]	Li YZ, Wu BJ, Yu YL, et al. Genome-wide analysis of the RING finger gene family in apple [J]. Mol Genet Genomics, 2011, 286(1): 81-94.
[16]	Qi ZY, Ahammed GJ, Jiang CY, et al. The E3 ubiquitin ligase gene SlRING1 is essential for plant tolerance to cadmium stress in Solanum lycopersicum [J]. J Biotechnol, 2020, 324: 239-247.
[17]	Tan B, Lian XD, Cheng J, et al. Genome-wide identification and transcriptome profiling reveal that E3 ubiquitin ligase genes relevant to ethylene, auxin and abscisic acid are differentially expressed in the fruits of melting flesh and stony hard peach varieties [J]. BMC Genomics, 2019, 20(1): 892.
[18]	Tang XL, Mei YY, He KX, et al. The RING-type E3 ligase RIE1 sustains leaf longevity by specifically targeting AtACS7 to fine-tune ethylene production in Arabidopsis [J]. Proc Natl Acad Sci USA, 2024, 121(48): e2411271121.
[19]	Xin TX, Zhang Z, Li S, et al. Genetic regulation of ethylene dosage for cucumber fruit elongation [J]. Plant Cell, 2019, 31(5): 1063-1076.
[20]	Wu T, Wang Y, Jin J, et al. Soybean RING-type E3 ligase GmCHYR16 ubiquitinates the GmERF71 transcription factor for degradation to negatively regulate bicarbonate stress tolerance [J]. New Phytol, 2025, 246(3): 1128-1146.
[21]	Shahwar D, Khan Z, Park Y. Molecular marker-assisted mapping, candidate gene identification, and breeding in melon (Cucumis melo L.): a review [J]. Int J Mol Sci, 2023, 24(20): 15490.
[22]	Liu YW, Gao YY, Chen MX, et al. GIFTdb: a useful gene database for plant fruit traits improving [J]. Plant J, 2023, 116(4): 1030-1040.
[23]	Li ML, Dong XY, Long GZ, et al. Genome-wide analysis of Q-type C₂H₂ ZFP genes in response to biotic and abiotic stresses in sugar beet [J]. Biology, 2023, 12(10): 1309.
[24]	Nystrom SL, McKay DJ. Memes: A motif analysis environment in R using tools from the MEME Suite [J]. PLoS Comput Biol, 2021, 17(9): e1008991.
[25]	Zhao DB, Gao FJ, Guan PY, et al. Identification and analysis of differentially expressed trihelix genes in maize (Zea mays) under abiotic stresses [J]. PeerJ, 2023, 11: e15312.
[26]	洪天澍, 海英, 恩和巴雅尔, 等. 甜瓜CmABCG8基因的表达特性分析 [J]. 生物技术通报, 2022, 38(7): 178-185.
	Hong TS, Hai Y, En H, et al. Analysis of expression characteristics of CmABCG8 gene in Cucumis melo L [J]. Biotechnol Bull, 2022, 38(7): 178-185.
[27]	Chen Q, Liu RJ, Wu YR, et al. ERAD-related E2 and E3 enzymes modulate the drought response by regulating the stability of PIP2 aquaporins [J]. Plant Cell, 2021, 33(8): 2883-2898.
[28]	Hao DD, Jin L, Wen X, et al. The RING E3 ligase SDIR1 destabilizes EBF1/EBF2 and modulates the ethylene response to ambient temperature fluctuations in Arabidopsis [J]. Proc Natl Acad Sci USA, 2021, 118(6): e2024592118.
[29]	Wang JY, Wang RY, Fang H, et al. Two VOZ transcription factors link an E3 ligase and an NLR immune receptor to modulate immunity in rice [J]. Mol Plant, 2021, 14(2): 253-266.
[30]	Wu Q, Zhang X, Peirats-Llobet M, et al. Ubiquitin ligases RGLG1 and RGLG5 regulate abscisic acid signaling by controlling the turnover of phosphatase PP2CA [J]. Plant Cell, 2016, 28(9): 2178-2196.
[31]	舒合凤, 胡荣美, 朱灵, 等. 水稻泛素连接酶基因RGLG31的同源克隆及表达分析[J/OL]. 分子植物育种, 2025. .
	Shu HF, Hu RM, Zhu L, et al. Homologous cloning and expression analysis of rice ubiquitin ligase gene RGLG31[J/OL]. Mol Plant Breed, 2025. .
[32]	Ma JF, Wang RB, Zhao HY, et al. Genome-wide characterization of the VQ genes in Triticeae and their functionalization driven by polyploidization and gene duplication events in wheat [J]. Int J Biol Macromol, 2023, 243: 125264.
[33]	Xu GX, Guo CC, Shan HY, et al. Divergence of duplicate genes in exon-intron structure [J]. Proc Natl Acad Sci USA, 2012, 109(4): 1187-1192.
[34]	Sun WQ, Li MD, Wang JB. Genome-wide identification and characterization of the RCI2 gene family in allotetraploid Brassica napus compared with its diploid progenitors [J]. Int J Mol Sci, 2022, 23(2): 614.
[35]	Nagels Durand A, Iñigo S, Ritter A, et al. The Arabidopsis iron-sulfur protein GRXS17 is a target of the ubiquitin E3 ligases RGLG3 and RGLG4 [J]. Plant Cell Physiol, 2016, 57(9): 1801-1813.
[36]	Zhang X, Wu Q, An CC. RGLG3 and RGLG4, novel ubiquitin ligases modulating jasmonate signaling [J]. Plant Signal Behav, 2012, 7(12): 1709-1711.
[37]	Zhang X, Wu Q, Ren J, et al. Two novel RING-type ubiquitin ligases, RGLG3 and RGLG4, are essential for jasmonate-mediated responses in Arabidopsis [J]. Plant Physiol, 2012, 160(2): 808-822.
[38]	陈佳琳. E3泛素连接酶CsRGLG4与转录因子CsAP2L调控柑橘果实采后绿霉病抗病性的机制研究 [D]. 重庆: 西南大学, 2024.
	Chen JL. Mechanism of E3 ubiquitin ligase CsRGLG4 and transcription factor CsAP2L in regulating postharvest green mold resistance in citrus fruits [D]. Chongqing: Southwest University, 2024.
[39]	Cheng MC, Hsieh EJ, Chen JH, et al. Arabidopsis RGLG2, functioning as a RING E3 ligase, interacts with AtERF53 and negatively regulates the plant drought stress response [J]. Plant Physiol, 2012, 158(1): 363-375.
[40]	Dong CH, Agarwal M, Zhang Y, et al. The negative regulator of plant cold responses, HOS1, is a RING E3 ligase that mediates the ubiquitination and degradation of ICE1 [J]. Proc Natl Acad Sci USA, 2006, 103(21): 8281-8286.

甜瓜CmRGLG基因家族鉴定及表达特性分析

Identification and Expression Characteristics Analysis of CmRGLG Gene Family in Melon

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