苹果锈果类病毒属属级寡核苷酸芯片筛查方法的建立

生物技术通报 ›› 2013, Vol. 0 ›› Issue (2): 151-156.

苹果锈果类病毒属属级寡核苷酸芯片筛查方法的建立

张永江¹ 辛言言¹ 朱水芳¹ 李世访²

（1. 中国检验检疫科学研究院，北京 100029; 2. 中国农业科学院植物保护研究所，北京 100193）

收稿日期:2012-08-01 修回日期:2013-02-27 出版日期:2013-02-26 发布日期:2013-02-27
作者简介:张永江，男，博士，副研究员，研究方向：植物检疫；E-mail ：zhangyjpvi@yeah.net
基金资助:
国家质检总局科技计划项目（2010IK278），质检公益性行业科研专项资助项目（201010256，20111035，201210214）

Establishment of the Screening Oligonucleotide Microarray of Apscaviroid in Genus Level

Zhang Yongjiang¹ Xin Yanyan¹ Zhu Shuifang¹ Li Shifang²

（1. Chinese Academy of Inspection and Quarantine，Beijing 100029 ；2. Institute of Plant Protection，CAAS，Beijing 100193）

Received:2012-08-01 Revised:2013-02-27 Published:2013-02-26 Online:2013-02-27

摘要/Abstract

摘要： 苹果锈果类病毒属是重要的植物类病毒属，目前尚无有效的筛查方法。通过对该属类病毒的核苷酸序列进行分析筛选，设计了35 条用于该属类病毒筛查的属级特异性探针并制备了寡核苷酸芯片。应用苹果锈果类病毒及柑橘矮化类病毒标准样品对该芯片进行验证，结果表明所建立的属级芯片可以获得有效的杂交信号，其灵敏度与RT-PCR 电泳法相当。该芯片可用于苹果锈果类病毒属类病毒的筛查，为该属类病毒的检疫与防控提供技术支撑。

关键词: 苹果锈果类病毒属, 属级探针, 寡核苷酸芯片

Abstract: Apscaviroid is a significant plant viroid genus and has not screening method available at present. For the establishment of the screening oligonucleotide microarray of Apscaviroid in genus level, thirty-five genus specific probes from the sequences of Apscaviroid viroid were designed. Microarray was constructed based on these probes and identificated with Apple scar skin viroid and Citrus dwarfing viroid standard samples. Results showed a high specificity for detection of Apscaviroid viroid and the same detection sensitivity with RT-PCR. The microarray can be used to screen and provide technical support of quarantine and control for Apscaviroid viroid.

Key words: Apscaviroid, Genus level probe, Oligonucleotide microarray

张永江, 辛言言, 朱水芳, 李世访. 苹果锈果类病毒属属级寡核苷酸芯片筛查方法的建立[J]. 生物技术通报, 2013, 0(2): 151-156.

Zhang Yongjiang, Xin Yanyan, Zhu Shuifang, Li Shifang. Establishment of the Screening Oligonucleotide Microarray of Apscaviroid in Genus Level[J]. Biotechnology Bulletin, 2013, 0(2): 151-156.

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