基于LAPS 系统的蛋白芯片表面修饰技术的对比研究

生物技术通报 ›› 2013, Vol. 0 ›› Issue (4): 215-220.

基于LAPS 系统的蛋白芯片表面修饰技术的对比研究

周爽¹, 贾芸芳², 孙晓轩¹, 邢克礼¹

1. 天津医科大学生物医学工程学院，天津 300070 ；2. 南开大学信息技术科学学院，天津 300071

收稿日期:2012-10-18 修回日期:2013-04-22 出版日期:2013-04-22 发布日期:2013-04-22
作者简介:周爽，硕士研究生，研究方向：生物信息检测与生物传感器；E-mail ：ispecter@163.com
基金资助:
天津市自然科学基金重点项目（12JCZDJC22400）

Comparative Study Based on the LAPS System of Protein Chip Surface Modification Technology

Zhou Shuang¹, Jia Yunfang², Sun Xiaoxuan¹, Xing Keli¹

1. Medical University of Tianjin School of Biomedical Engineering, Nankai University, Tianjin 300070 ； 2. College of Information Technical Science, Tianjin 300071

Received:2012-10-18 Revised:2013-04-22 Published:2013-04-22 Online:2013-04-22

摘要/Abstract

摘要： 采用3-氨基丙基-三甲氧基硅烷（（3-aminopropy1）trimethoxysilane，APTES）和戊二醛（glutaraldehyde，GA）、左旋多巴（L-DOPA）修饰芯片载体表面，对两种不同修饰方法制备的蛋白质芯片进行对比研究。以XPS 检测表面活化剂的修饰效果；将AFP 抗体固定在修饰后的片基上，加入AFP 溶液，以蛋白探针的反应性作为监测指标，通过LPAS 系统检测反应产生的光电流。结果显示，左旋多巴修饰硅片对蛋白固定效果较好，有较高的反应活性，检测范围较宽，但幅值较小。

关键词: LAPS, 蛋白芯片, 表面修饰

Abstract: The surface of chips were modified by 3 - aminopropyl - trimethoxysilane（（3-aminopropy1）trimethyoxysilane APTES）and glutaraldehyde（Glutaraldehyde, GA）, levodopa（L-DOPA）modification of the chip carrier surface. The two kinds of different types of modification of the protein chips prepared a comparative study. XPS detect surface modification effect of the activator. AFP antibodies were fixed on the modified film base, then adding AFP solution, and photocurrent detected by LPAS system, selecting reactive protein probes as monitoring indicators. The results showed the silicon protein modified by levodopa fixing preferably, having a higher reaction activity and a large detection limit, though the amplitudes were small.

Key words: LAPS, Protein chip, Surface modification

周爽, 贾芸芳, 孙晓轩, 邢克礼. 基于LAPS 系统的蛋白芯片表面修饰技术的对比研究 [J]. 生物技术通报, 2013, 0(4): 215-220.

Zhou Shuang, Jia Yunfang, Sun Xiaoxuan, Xing Keli. Comparative Study Based on the LAPS System of Protein Chip Surface Modification Technology[J]. Biotechnology Bulletin, 2013, 0(4): 215-220.

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