河西绒山羊GOLA-DQA1基因第2外显子遗传特征分析

生物技术通报 ›› 2013, Vol. 0 ›› Issue (5): 121-125.

河西绒山羊GOLA-DQA1基因第2外显子遗传特征分析

柴文琼, 王继卿, 胡江, 刘秀, 李少斌, 罗玉柱

(甘肃农业大学甘肃省草食动物生物技术重点实验室动物科学技术学院研究测试中心，兰州 730070)

收稿日期:2012-11-23 修回日期:2013-05-24 出版日期:2013-05-24 发布日期:2013-05-24
作者简介:柴文琼，女，硕士研究生，研究方向：动物遗传育种与繁殖；E-mail：358215320@qq.com
基金资助:
甘肃省省青年科技基金计划(1107RJYA060)，2012年甘肃省高等学校基本科研业务费项目(GSAU-STS-1231)，甘肃农业大学盛彤笙科技创新项目(GAU-YZ1007)，2012年甘肃省农业科技创新项目

Genetic Characteristics of GOLA-DQA1 Gene at Exon2 in Hexi Cashmere Goat

Chai Wenqiong, Wang Jiqing, Hu Jiang, Liu Xiu, Li Shaobin, Luo Yuzhu

(Gansu Key Laboratory of Herbivorous Animal Biotechnology，Faculty of Animal Sci-tech，Analysis and Research Center of Gansu Agricultural University，Lanzhou 730070)

Received:2012-11-23 Revised:2013-05-24 Published:2013-05-24 Online:2013-05-24
About author:罗玉柱，男，教授，博士生导师，研究方向：生物技术与动物育种；E-mail：luoyz@gsau.edu.cn

摘要/Abstract

摘要： 采用PCR-SSCP和测序技术，分析864只河西绒山羊(有流产表型记录)GOLA-DQA1基因的遗传特性及其与山羊流产的关联性。研究结果表明，在河西绒山羊DQA1基因第2外显子上检测到9个等位基因，其中GOLA-DQA1*B、GOLA-DQA1*E和GOLA-DQA1*H 3个等位基因为本研究首次发现。9个等位基因中存在42个核苷酸多态位点，占分析位点总数的12.65%，包括23个转换位点，13个颠换位点，5个转换和颠换共存位点及1个插入位点。相关性分析结果表明，不同表型间的等位基因分布差异显著(P<0.05)，病例组中等位基因GOLA-DQA1*A的频率显著高于正常组(P<0.05)，等位基因GOLA-DQA1*H的频率显著低于正常组(P<0.05)。由此推测等位基因GOLA-DQA1*A与山羊流产的易感性相关，GOLA-DQA1*H与流产抗性相关。

关键词: GOLA-DQA1基因, 河西绒山羊, 遗传特征, PCR-SSCP, 流产

Abstract: Genetic Characteristics of GOLA-DQA1 gene at exon2 and the association of alleles with pregnancy in Hexi cashmere goat were analyzed using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and sequencing technology. Nine alleles were identified at GOLA-DQA1 gene at exon2 in Hexi cashmere goat and 3 new DQA1 alleles were identified (GOLA-DQA1*B, GOLA-DQA1*E and GOLA-DQA1*H). Sequence analysis indicated that there were 42 single nucleotide polymorphism sites, which occupied 12.65% of exon 2 sequences which include twenty three transition sites, thirteen transversion sites, five coexist sites and one insertion site. Correlation analysis showed significant statistic difference between different alleles (P<0.05). Frequencies of GOLA-DQA1*A were significantly in abortion group higher than those in normal group (P<0.05). In contrast, GOLA-DQA1*H allelic frequencies in abortion group were significance lower than those in normal group (P<0.05). The result suggested that the allele GOLA-DQA1*A might associated with abortion and GOLA-DQA1*H might related to pregnancy protection in Hexi Cashmere Goat.

Key words: GOLA-DQA1 gene, Hexi cashmere goat, Genetic characteristic, PCR-SSCP, Abortion

柴文琼, 王继卿, 胡江, 刘秀, 李少斌, 罗玉柱. 河西绒山羊GOLA-DQA1基因第2外显子遗传特征分析[J]. 生物技术通报, 2013, 0(5): 121-125.

Chai Wenqiong, Wang Jiqing, Hu Jiang, Liu Xiu, Li Shaobin, Luo Yuzhu. Genetic Characteristics of GOLA-DQA1 Gene at Exon2 in Hexi Cashmere Goat[J]. Biotechnology Bulletin, 2013, 0(5): 121-125.

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(责任编辑马鑫)