烟草赤星病菌拮抗性放线菌Q14的筛选及鉴定

生物技术通报 ›› 2013, Vol. 0 ›› Issue (10): 127-130.

烟草赤星病菌拮抗性放线菌Q14的筛选及鉴定

廉立慧,高丽君,杨娜,王涛,张显忠

（泰山医学院，泰安 271000）

收稿日期:2013-03-25 修回日期:2013-10-15 出版日期:2013-10-14 发布日期:2013-10-15
作者简介:廉立慧，女，硕士，讲师，研究方向：微生物学；E-mail ：18200038@qq.com
基金资助:
泰山医学院面上基金项目

Screening and Identification of Actinomyces Q14 Antagonistic Against Alternaria Alternata

Lian Lihui, Gao Lijun, Yang Na, Wang Tao, Zhang Xianzhong

（Taishan Medical College，Taian 271000）

Received:2013-03-25 Revised:2013-10-15 Published:2013-10-14 Online:2013-10-15

摘要/Abstract

摘要：

烟草赤星病对烟草生产威胁极大，旨在通过筛选拮抗菌为烟草赤星病菌的生物防治提供新的选择。采用平板分离培养、对峙平板法、16S rDNA 系统发育分析等方法。结果显示，从茄子根围土壤中分离到36 株放线菌，以烟草赤星病菌为目标菌，采用对峙平板法测定，筛选到6 株有拮抗活性菌株，占所分离总数的16.67% 。其中Q14 对烟草赤星病菌的拮抗作用最强，抑菌带宽为14.5 mm，并且对黄瓜枯萎病菌、番茄枯萎病菌也有较强的拮抗作用。通过对菌株Q14 的形态观察培养特征生理生化反应和16S rDNA 测定（GenBank 登录号KC675186），将其鉴定为多产色链霉菌。分离获得的一株多产色链霉菌拮对烟草赤星病有较好的拮抗作用，目前国内尚属首次报道，对烟草赤星病生物防治又提供了新的选择。

关键词: 烟草赤星病菌, 拮抗性放线菌, 多产色链霉菌

Abstract:

It was to screen the new antagonistic actinomyces against Alternaria alternata. Totally 36 strains of actinomycetes were obtained from eggplant roots. By dual culture on PDA plates, it was found that 6 strains（16.67% of total isolates）of actinomycetes showed satisfactory antagonistic effects to Alternaria alternate. Among them, strain Q14 exhibited the strongest antibiosis capacity with an inhibitory zone of 14.5 mm, and also showed good antagonistic effects to Fusarium oxysporum f. sp. cucumerinum and Fusarium oxysporum f. sp. lycopersici.
Strain Q14 was identified as Streptomyces polychromogenes according to its 16S rDNA sequence（GenBank acc. No. KC675186）analysis, and the morphological, physiological and biochemical characteristics.

Key words: Alternaria alternate, Antagonistic actinomycetes, Streptomyces polychromogenes

廉立慧,高丽君,杨娜,王涛,张显忠. 烟草赤星病菌拮抗性放线菌Q14的筛选及鉴定[J]. 生物技术通报, 2013, 0(10): 127-130.

Lian Lihui Gao Lijun Yang Na Wang Tao Zhang Xianzhong . Screening and Identification of Actinomyces Q14 Antagonistic Against Alternaria Alternata[J]. Biotechnology Bulletin, 2013, 0(10): 127-130.