关键词: Act0988拮抗菌株, 鉴定, 多产色链霉菌, 培养条件, 生长

Abstract: Strain Act0988 produces the metabolite with antifungal activity against many kinds of mould,such as Penicillium italicum et al. The strain identification and study of the relationship between cultivation condition and the strain growth were aimed at laying the foundation for the screening media and research of fermentation processes during the development of the metabolite in future. The strain was identified by its morphological and culture characteristics,physiological and biochemical characteristics and 16S rDNA sequencing. The relationships between carbon sources,nitrogen sources,temperature,pH,oxygen and strain growth were studied with shake flask cultivation. Strain Act0988 was identified as Streptomyces polychromogenes,the biomass of fermentative broth was 5.8 mg/mL if the optimal carbon source was soluble starch,the biomass was about 5.7 mg/mL while yeast extract and peptone were the optimal nitrogen sources,the biomass was 6.0 mg/mL when the optimal temperature was 28℃,the biomass was 6.2 mg/mL while the most suitable initial pH was 7.0,and the biomass was 6.3 mg/mL if the optimal volume of the medium was 60 mL per 500 mL shake flask. For being easy to be cultivated and fermentation broth owing high antifungal activity,the metabolites produced by strain Act0988 has the great potential of development and utilization.

Key words: antagonistic strain Act0988, identification, Streptomyces polychromogenes, cultivation conditions, growth