解脂耶氏酵母诱变育种及发酵产α-酮戊二酸条件优化

生物技术通报 ›› 2014, Vol. 0 ›› Issue (7): 190-195.

解脂耶氏酵母诱变育种及发酵产α-酮戊二酸条件优化

巩健^{1, 2}

1. 淄博职业学院制药与生物工程系, 淄博 255314；
2. 山东省曲霉应用工程技术研究中心, 淄博 255314

收稿日期:2013-12-25 出版日期:2014-07-15 发布日期:2014-07-16
基金资助:
国家自然科学基金项目（31260396）, 淄博市科学技术发展计划项目（2010GG01124, 2011GG01243）

Mutation Breeding of Yarrowia lipolytica and Optimization of α-Ketoglutaric Acid Fermentation Conditions

Gong Jian^{1, 2}

1. Pharmaceutical & Biological Engineering Department, Zibo Vocational Institute, Zibo 255314；
2. Shandong Provincial Engineering Center on Aspergillus Application, Zibo 255314

Received:2013-12-25 Published:2014-07-15 Online:2014-07-16

摘要/Abstract

摘要： 对具有发酵产α-酮戊二酸能力的解脂耶氏酵母（Yarrowia Lipolytica）ZY-4进行了紫外诱变和NTG诱变育种, 筛选得到产量提高的突变株, 并对突变株的发酵培养基进行了优化, 结果表明, 紫外诱变和NTG诱变后筛选到的突变株分别比原始出发菌株产量提高了67.8%和110%。优化后发酵培养基成分为甘油 8%, 氯化铵5.0 g/L, 硫胺素1.0 μg/L, 磷酸二氢钾 1.0 g/L, 七水硫酸镁 0.5 g/L, 培养基优化后α-酮戊二酸产量比原始出发菌株提高了232.4%。

关键词: α-酮戊二酸, 解脂耶氏酵母, 诱变育种

Abstract: A strain of Yarrowia lipolytica（ZY-4）which producing α-ketoglutaric acid（KGA）was treated for mutation by UV and NTG. Mutation strains which could accumulate much more KGA were obtained. Culture medium for mutation strain on KGA fermentation was also studied. The concentration of KGA accumulated by UV mutation strain and NTG mutation strain increased 67.8% and 110% respectively compared with original strain. The optimal fermentation medium contains glycerol 8%, NH₄Cl 5.0 g/L, thiamine 1.0 μg/L, KH₂P0₄ 1.0 g/L and MgSO₄·7H 2O 0.5 g/L. The production of KGA increased 232.4% after optimization when compared to the original strain.

Key words: α-ketoglutaric acid, Yarrowia lipolytica, Mutantion Breeding

巩健. 解脂耶氏酵母诱变育种及发酵产α-酮戊二酸条件优化[J]. 生物技术通报, 2014, 0(7): 190-195.

Gong Jian. Mutation Breeding of Yarrowia lipolytica and Optimization of α-Ketoglutaric Acid Fermentation Conditions[J]. Biotechnology Bulletin, 2014, 0(7): 190-195.

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